结直肠癌组织中GPR116的表达及其临床意义

Expression of GPR116 in colorectal carcinoma tissues and its clinical significance

目的:探讨结直肠腺癌组织中G蛋白偶联受体116(G-protein-coupled receptor 116,GPR116)的表达及其临床意义。方法:采用Western blot检测8例结直肠腺癌新鲜组织及配对正常肠黏膜组织中GPR116表达情况,采用免疫组化法检测46例石蜡包埋结直肠腺癌组织中GPR116的表达。结果:Western blot显示,GPR116在8例结直肠腺癌组织中的表达显著高于正常肠黏膜组织(P=0.010)。免疫组化染色显示,在46例结直肠腺癌组织中,8例(17.4%)GPR116低表达,38例(82.6%)高表达;同一蜡块中癌周的非癌组织中43例(93.5%)GPR116低表达,3例(6.5%)高表达;GPR116在癌组织中的表达高于同一蜡块中癌周的非癌组织,差异有统计学意义(P=0.000)。p53阳性病例GR116高表达率(66.7%,16/24)低于p53阴性病例(100.0%,17/17)(P=0.024)。GPR116的表达水平与患者年龄、性别、肿瘤位置、肿瘤大小、T分期、组织学分级、淋巴结是否转移无统计学意义的相关性(P>0.05)。GPR116低表达组与高表达组病例生存时间无明显差异(P=0.203)。结论:在结直肠癌组织中GPR116的表达上调,GPR116的表达与p53的表达呈负相关趋势。

Objective:To examine the expression and the clinical significance of GPR116 in colorectal carcinoma(CRC)tissues.Methods:We performed Western blot to assess the expression level of GPR116 in 8 cases of fresh CRC tissues and the corresponding normal tissues,and used immunohistochemistry(IHC)to detect the expression of GPR116 in 46 cases of paraffin-embedded CRC tissues.Results:Western blot showed the expression of GPR116 was significantly higher in CRC tissues compared with the corresponding normal tissues(P=0.010).IHC showed that in 46 CRC tissues,8 cases(17.4%)had low expression of GPR116 and 38 cases(82.6%)had high expression.And in the non-cancer tissues adjacent CRC tissues in the same paraffin block,43 cases(93.5%)had low expression of GPR116 and 3 cases(6.5%)had high expression.The expression of GPR116 in CRC tissues was higher than that in non-cancer tissues adjacent CRC in the same paraffin block,and the difference was statistically significant(P=0.000).The GPR116-high expression rate in p53 positive cases(66.7%,16/24)was lower than that in p53 negative cases(100.0%,17/17)(P=0.024).The expression of GPR116 was not related to the patients'age,gender,mass location,tumor size,T stage,histological grade and lymph node metastasis(P>0.05).The overall survival analysis showed there was no significance between GPR116-low expression group and GPR116-high expression group(P=0.203).Conclusion:The expression of GPR116 was up-regulated in CRC tissues,and there was a negative correlation trend between the expression of GPR116 and the expression of p53 in CRC.