急性髓系白血病中SMC3基因的表达及意义

Expression and significance of SMC3 gene in acute myeloid leukemia

目的:探讨急性髓系白血病(acute myeloid leukemia,AML)患者中SMC3基因表达的临床意义。方法:收集本院急性髓系白血病患者49例、缺铁性贫血(iron deficiency anemia,IDA)患者20例。应用实时荧光定量PCR(real-time quantitative PCR,qRT-PCR)法检测骨髓细胞中SMC3(structural maintenance of chromosome 3)mRNA表达,将患者分为高表达组和低表达组,分析SMC3表达水平与患者临床特征和预后分组之间的关系。结果:49例AML患者骨髓细胞标本SMC3 mRNA相对表达量高于对照组,SMC3基因表达与ELN(European Leukemia Net)AML遗传学预后分组相关(P=0.011),高表达组中位生存时间4个月,低表达组中位生存时间为19个月,结果具有统计学显著性差异(P=0.000)。SMC3表达与年龄、性别、FAB分型、骨髓原始细胞、白细胞、血红蛋白、血小板计数均无统计学差异(P>0.05)。结论:SMC3 mRNA高表达与AML不良预后相关,作为可供筛选候补预后指标,其预后价值有待于在大样本研究中进一步验证。

Objective:To explore the clinical significance of SMC3 gene expression in patients with acute myeloid leukemia(AML).Methods:Cases of 49 patients with acute myeloid leukemia and 20 patients with iron deficiency anemia(IDA)were collected.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of SMC3(structural maintenance of chromosome 3)mRNA in bone marrow cells.The patients were divided into high expression group and low expression group.Relationship between the expression of SMC3 and prognostics was analyzed.Results:The relative expression of SMC3 mRNA in bone marrow cell samples from 49 AML patients was higher than that of the control group.The expression of SMC3 gene was correlated with ELN(European Leukemia Net)AML genetic prognosis group(P=0.011).The median survival time of the high expression group was 4 momths.The median survival time of the low expression group was 19 months,and the results were statistically significant(P=0.000).There were no significant differences in SMC3 expression with age,gender,FAB typing,bone marrow blasts,white blood cells,hemoglobin and platelet counts(P>0.05).Conclusion:High expression of SMC3 mRNA is associated with poor prognosis of AML.As a candidate prognostic indicator for screening,it needs to be further verified in large sample studies.