新疆驴细管冻精的制作

Production of Xinjiang donkey straw frozen semen

试验旨在研究新疆驴细管冻精制作过程中精液冷冻液、降温平衡时间及液氮熏蒸时间对精子冻后活力的影响。研究共设计3个试验。试验1对比了INRA-Freeze、Optidyl、房氏驴精液冷冻保存液、自配马精液冷冻冷冻液等4种精液冷冻液在精子冷冻过程对精子冻后活力的影响,筛选出适合制作新疆驴细管冻精的冷冻液;试验2探索在细管冻精制作过程中平衡90、120、150 min对精子冻后活力的影响;试验3比较在细管冻精制作过程中使用液氮熏蒸5、6、7、8、9、10 min时对精子冻后活力的影响,探索液氮熏蒸的最佳时间。结果显示,使用INRA-Freeze冷冻液或房氏冷冻液在冷冻过程中对精子的损伤最小;冷冻过程中,降温平衡90、120、150 min对精子冻后活力的影响差异不显著(P>0.05);在冷冻过程中液氮熏蒸7 min时精子冻后活力最高,显著高于熏蒸5、9、10 min的精子冻后活力(P<0.05),与熏蒸6、8 min时精子冻后活力差异不显著(P>0.05)。研究表明,为降低成本可使用INRA-Freeze冷冻液稀释精液,于冰箱中平衡90~150 min后装入0.5 mL的细管中、液氮熏蒸7 min制作的冻精细管的精子活力最佳。

The experiment was conducted to study the effect of semen freezing liquid,cooling equilibrium time and liquid nitrogen fumigation time on sperm viability after freezing during the production of straw frozen semen of Xinjiang donkey.Three experiments were designed.In experiment 1,the effect of INRA-Freeze,Optidyl,Fang's donkey semen cryopreservation solution and self-matching horse semen cryopreservation solution on sperm viability during sperm freezing were compared,and the suitable cryopreservation solution for Xinjiang donkey straw cryopreservation was selected.In experiment 2,the effect of balance of 90,120 and 150 min on sperm viability after freezing were investigated.In experiment 3,the effect of liquid nitrogen fumigation for 5,6,7,8,9 and 10 min on sperm viability after freezing were compared to explore the optimal time of liquid nitrogen fumigation.The results showed that the use of INRA-Freeze or Fang's donkey semen cryopreservation caused the least damage to sperm during freezing.During the freezing process,the effect of cooling equilibrium for 90,120,and 150 min on the sperm motility after freezing were not significantly different(P>0.05).During the freezing process,the sperm motility after freezing was the highest when fumigated with liquid nitrogen for 7 min,which was significantly higher than that after fumigation for 5,9,and 10 min(P<0.05),the sperm motility after freezing was not significantly different from that of 6 and 8 min fumigation(P>0.05).The study indicates that in order to reduce the cost,the semen can be diluted with INRA-Freeze freezing solution.The sperm motility was the best in the frozen fine tubes prepared by equilibrating in the refrigerator for 90~150 min,then placing them into 0.5 mL straws and fumigating with liquid nitrogen for 7 min.