止抽汤对抽动障碍模型大鼠抽动行为及TNF-α、IL-6、IL-1β的影响

Effects of Zhichou Decoction on Tic Behavior and TNF-α、IL-6、IL-1βof Tic Disorder Model Rats

目的观察止抽汤对抽动障碍(tic disorder,TD)模型大鼠抽动行为及血清、纹状体TNF-α、IL-6、IL-1β的影响,探讨其作用机制。方法Wistar大鼠腹腔注射亚氨基二丙腈(IDPN)造模成功后,将其随机分为模型组、止抽汤组和盐酸硫必利组,并设立正常对照组。各组予相应药物灌胃14 d,于第7天、14天进行抽动行为评分,末次行为评分后,用酶联免疫法(ELISA)检测大鼠血清TNF-α、IL-6、IL-1β水平,荧光定量PCR、免疫印迹法(Western-blot)分别检测大鼠纹状体TNF-α、IL-6、IL-1βmRNA及蛋白表达水平。结果①药物灌胃7 d后,止抽汤组、盐酸硫必利组与模型组比较,刻板行为评分明显降低(P<0.01),运动行为评分无统计学意义(P>0.05)。灌胃治疗14 d后,与模型组比较,止抽汤组、盐酸硫必利组运动行为评分及刻板行为评分显著降低(P<0.01)。②止抽汤组、盐酸硫必利组大鼠血清及纹状体TNF-α、IL-6、IL-1β表达水平明显低于模型组(P<0.01)。结论止抽汤能明显改善模型大鼠的运动行为及刻板行为,其机制可能与降低TD大鼠血清及纹状体TNF-α、IL-6、IL-1β的高表达有关。

Objective To observe the effect of Zhichou Decoction on the tic behavior and the expression of TNF-α,IL-6 and IL-1βin serum and striatum of rats in tic disorder(tic disorder,TD),and to explore its mechanism.Methods The model was made by intraperitoneal injection of iminodipropionitrile(IDPN),and after the model group was established,the rats were randomly divided into model group,Zhizhou Decoction group and thiapride hydrochloride group,and a blank control group was established.Each group was given 14 days of corresponding drug-gavage intervention.After 7 and 14 days of drug intervention,rat behavior score was performed.After the last behavior score,rat serum TNF-α,IL-6 and IL-1βlevel was detected by ELISA method and the expression levels of TNF-α,IL-6 and IL-1βmRNA and protein in rat striatum were measured by fluorescence quantitative PCR and immunoblotting,respectively.Results①After 7 days of drug gavage,compared with the control group,the scores of rigid behavior were significantly decreasedin the zhichou decoction group and thiapride hydrochloride group(P<0.01),and the motor behavior score was not statistically significant(P>0.05).After 14 days of drug gavage,compared with the control group,the scores of rigid behavior and motor behavior were significantly decreasedin the zhichou decoction group and thiapride hydrochloride group(P<0.01).②Serum and striatum TNF-α,IL-6 and IL-1βexpression levels were compared:Zhichou Decoction group and thiapride hydrochloride group were significantly lower than control group(P<0.01).Conclusion Zhichou Decoction can obviously improve the motor behavior and stereotype behavior of the model rats,and its mechanism may be related to the decrease of serum、striatum TNF-α,IL-6 and IL-1βexpression level.