急性心肌缺血模型大鼠内关、心俞穴穴区肥大细胞的分布及特征

Characteristics and Distribution of Mast Cells at Neiguan and Xinshu Acupoints of Acute Myocardial Ischemia Rats

目的观察急性心肌缺血模型大鼠不同穴区肥大细胞(MC)活化情况。方法健康SD雄性大鼠12只,随机分为对照组、模型组各6只。皮下多点位注射盐酸异丙肾上腺素(ISO)制备大鼠急性心肌缺血模型,氯化三苯基四氮唑(TTC)染色法测定心肌缺血面积验证造模成功与否,甲苯胺蓝染色法检测左侧心俞穴、左侧内关穴穴区MC分布及活化情况。结果①模型组心肌缺血面积明显大于对照组(P<0.05);②与对照组相比,模型组左侧心俞穴MC数目和MC脱颗粒率显著增大(P<0.05);左侧内关穴MC脱颗粒率升高显著(P<0.05),MC数目没有显著差异;③对照组左侧内关穴MC数目大于左侧心俞穴MC数目(P<0.05),模型组左侧内关穴与心俞穴MC数目没有明显差异。结论急性心肌缺血可引起心俞穴和内关穴穴位敏化,穴区呈现MC募集和脱颗粒活化表达;正常状态下不同部位穴位MC含量不同,推测四肢部位腧穴MC含量高于背部腧穴。

Objective To explore the activation of mast cells in the different acupoints area of acute myocardial ischemia rats.Methods Twelve healthy male SD rats were stochastically assigned to control group and model group,with 6 rats per group.Acute myocardial ischemia was produced in rats by injecting 60 mg/kg of isoproterenol subcutaneously at multiple points.Myocardial ischemia proportion was measured by triphenyltetrazolium chloride(TTC)staining to verify the success of the model.Distribution and activation of mast cells in the left Neiguan(PC6)and in the left Xinshu(BL15)of rats was detected by toluidine blue staining.Results 1.The myocardial ischemia area of model group was significantly higher than it of control group(P<0.05);2.Compared with the control group,the number and degranulation rate of MCs in the left Xinshu of model group were significantly increased(P<0.05);the degranulation rate of MCs in the left Neiguan of model group was significantly increased(P<0.05),there was no significant difference in the number of MCs in the left Neiguan between 2 groups;3.The number of MC in the left Neiguan was higher than that in the left Xinshu among control group(P<0.05),no significant difference in the number of MCs between that 2 acupoints among model group.Conclusion Xinshu(BL15)and Neiguan(PC6)in the acute myocardial ischemia model rats were sensitized,which displayed visible recruitment and degranulation of MCs;The number of MCs was different in acupoints of different body parts,that in limbs acupoints probably higher than that in back acupoints.