原发性胆囊癌中T淋巴瘤侵袭转移诱导因子1的表达及临床意义

Expression of Tiam1 in primary gallbladder cancer and its clinical significance

目的:探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)在原发性胆囊癌中的表达及其与临床病理特征的相互关系和临床意义。方法:收集2012年1月—2018年1月经病理证实为原发性胆囊癌患者的石蜡标本52例及胆囊良性疾病患者石蜡标本10例,均为手术后切除标本,并且临床资料完整。对62例胆囊组织标本进行Tiam1的免疫组织化学方法检测,在高倍显微镜下观察结果。采用SPSS 18.0统计软件,结合患者临床资料分析其生物学特征及临床意义。结果:52例原发性胆囊癌患者中Tiam1阳性表达44例(84.61%),但在非肿瘤性胆囊组织中没有表达。Tiam1在淋巴结转移(P=0.010)、淋巴血管侵犯(P=0.009)和复发(P=0.010)的原发性胆囊癌组织中表达阳性。另外,Tiam1的阳性表达与肿瘤的浸润深度(P=0.007)和TNM分期(P=0.005)明显相关,与性别、年龄、肿瘤大小及肿瘤部位无明显关系(P>0.05)。结论:Tiam1在原发性胆囊癌中表达明显高于非肿瘤性胆囊组织,并与患者的淋巴结转移、复发、肿瘤浸润深度和TNM分期有关,与年龄、性别、肿瘤大小无关。Tiam1可能是胆囊癌患者预后不良的一项独立预测因素。

Objective: To study the expression of T lymphoma invasion and metastasis inducing factor 1(Tiam1),analyze the relationship and significance between the expression and clinicopathological characteristics.Methods: Fifty-two paraffin specimens from patients with pathologically confirmed primary gallbladder cancer from January 2012 to January 2018 and 10 paraffin specimens from patients with benign gallbladder disease were collected as controls, all of which were resected specimens after surgery, and the clinical data were complete.Immunohistochemical detection of Tiam1 was performed on 62 gallbladder tissue samples, and the results were observed under a high magnification microscope.SPSS 18.0 statistical software was used to analyze the biological characteristics and clinical significance of the patients combined with clinical data.Results: The positive rate of Tiam1 was 84.61%(44 cases) in 52 cases of primary gallbladder carcinoma, and negative in non-neoplastic gallbladder tissue.The expression of Tiam1 was higher in lymph node metastasis(P=0.010),lymphatic metastasis(P=0.009) and recurrence(P=0.010) in primary gallbladder carcinoma.In addition, the expression of Tiam1 was significantly relative with the depth of tumor invasion(P=0.007) and TNM stage(P= 0.005) and no relation with gender, age, tumor size and tumor location(P>0.05).Survival time of Tiam1 positive patients was shorter than those negative by Kaplan-Meier survival curve(P=0.010).Conclusion: Tiam1 expression of primary gallbladder cancer was significantly higher than non-neoplastic gallbladder tissue, and it was related with the recurrence, tumor invasion depth and TNM stage.Tiam1 maybe is an independent predictive factor of.primary gallbladder cancer patients.