槐耳清膏对免疫性血小板减少症患者B淋巴细胞的作用研究

Effect of Huaier extract on B lymphocytes and their subsets in patients with immune thrombocytopenia purpura

目的:探究槐耳清膏对免疫性血小板减少症(immune thrombocytopenic purpura,ITP)患者B淋巴细胞及其亚群的作用。方法:分析2019年1月至2020年3月大连医科大学附属第二医院诊断的20例ITP患者的外周血,患者中位年龄53.5(16.0~87.0)岁,其中男性8例,女性12例。首先采用流式细胞术比较患者与健康对照者CD19^(+)B淋巴细胞及其亚群CD19^(+)CD27^(+)记忆B淋巴细胞、CD19^(+)CD24^(+)CD38^(+)调节B淋巴细胞比例异同;用不同浓度(2、4、6 mg·mL^(-1))槐耳清膏处理B淋巴细胞株,应用CCK-8检测槐耳清膏对B淋巴细胞增殖的影响;流式细胞仪测定槐耳清膏处理外周血72 h后B淋巴细胞亚群的变化。结果:ITP患者CD19^(+)B淋巴细胞比例明显高于正常对照;中药槐耳清膏能够抑制B淋巴细胞的增殖,抑制ITP患者记忆性B淋巴细胞及调节B淋巴细胞。结论:中药槐耳清膏能抑制ITP患者B淋巴细胞的增殖,本研究为临床的应用提供理论依据。

Objective:To investigate the effects of Huaier extract on B lymphocytes and their subsets in patients with immune thrombocytopenia purpura(ITP).Methods:The peripheral blood of 20 patients with ITP diagnosed in The Second Affiliated Hospital of Dalian Medical University from January 2019 to March 2020 were analyzed.The median age was 53.5(16.0-87.0)years,including 8 males and 12 females.CD19^(+)B-lymphocytes and their subsets,CD19^(+)CD27^(+)memory B and CD19^(+)CD24^(+)CD38^(+)regulatory B cells,were compared between patients with ITP and normal controls using flow cytometry.Next,cells from a B-lymphocyte line were treated with different concentrations(2,4,6 mg•mL-1)of Huaier extract.CCK-8 was used to assess the effect of Huaier extract on B-lymphocyte proliferation.Changes in B-lymphocyte subsets in peripheral blood treated with Huaier extract for 72 h were determined using flow cytometry.Results:The proportion of CD19^(+)B-lymphocytes was significantly higher in patients with ITP than in normal controls.Huaier extract can inhibit the proliferation of B-lymphocytes,as well as the proliferation of regulatory B cells and memory B cells in patients with ITP.Conclusions:Huaier extract can inhibit the proliferation of B-lymphocytes in patients with ITP,providing a possible theoretical basis for clinical application.