肝细胞癌的细胞分裂周期基因20表达与临床病理特征关系及对预后的影响

The relationship between the expression of cell division cycle gene 20 in hepatocellular carcinoma and the clinicopathological characteristics of patients and its influence on prognosis

目的分析肝细胞癌病人细胞分裂周期基因20(CDC20)表达与临床病理特征的关系及对预后的影响。方法收集2016年5月至2017年12月在商丘市第三人民医院行手术治疗的肝细胞癌病人146例,实时荧光定量逆转录聚合酶链反应(qRT-PCR)、蛋白质印迹法和免疫组织化学检测肝细胞癌及癌旁组织中CDC20的表达情况。将免疫免疫组织化学评分0~7分和8~12分的病人分别设为CDC20低水平组(n=86)和CDC20高水平组(n=60),分析两组间的临床病理特征和生存率。结果CDC20 mRNA在肝细胞癌组织中相对表达水平为(2.33±0.67),高于癌旁组织(0.97±0.29)(P<0.001)。CDC20在肝细胞癌组织中蛋白表达水平为(0.91±0.05),高于癌旁组织(0.14±0.05)(P<0.001)。CDC20在肝细胞癌组织中阳性表达率为77.4%(113/146),高于癌旁组织8.2%(12/146)(P<0.001)。CDC20的表达与病人的肿瘤长径、被膜侵犯、脉管侵犯、肿瘤多发、分化程度和TNM分期有关(均P<0.05)。CDC20高水平组术后3年总生存率(61.7%)和疾病无进展生存率(50.0%),均低于CDC20低水平组(87.2%、69.8%)(均P<0.05)。结论肝细胞癌中CDC20的水平增加,可能参与肿瘤的增殖和侵袭。CDC20高水平提示病人的不良预后,可能成为肝细胞癌预后判断的标志物。

Objective To analyze the relationship between the expression of hepatocellular carcinoma cell division cycle gene 20(CDC20)and clinicopathological characteristics of patients and its impact on prognosis.Methods A total of 146 hepatocellular carcinoma patients who underwent surgical treatment in Shangqiu Third People's Hospital from May 2016 to December 2017 were collected.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),Western blotting and immunohistochemistry were used to detect the expression of CDC20 in hepatocellular carcinoma and adjacent tissues.The patients with immunohistochemical score of 8-12 points were enrolled in CDC20 high leave group(n=60),and the patients with immunohistochemical score of 0-7 points were enrolled in CDC20 low leave group(n=86).The clinicopathological features and survival rates of the two groups were analyzed.Results The relative expression level of CDC20 mRNA in hepatocellular carcinoma tissues was(2.33±0.67),which was higher than that of adjacent tissues(0.97±0.29),(P<0.001).The protein expression level of CDC20 in hepatocellular carcinoma tissue was(0.91±0.05),which was higher than that of adjacent tissues(0.14±0.05)(P<0.001).The positive expression rate of CDC20 in hepatocellular carcinoma tissues was 77.4%(113/146),which was higher than that in adjacent tissues 8.2%(12/146)(P<0.001).The high expression of CDC20 was related to the patient's maximum tumor diameter,capsule invasion,vascular invasion,tumor frequency,degree of differentiation and TNM stage(all P<0.05).The 3-year overall survival rate(61.7%)and disease progression-free survival rate(50.0%)in the CDC20 high leave group were lower than those in the CDC20 low leave group(87.2%,69.8%)(All P<0.05).Conclusions The expression level of CDC20 in hepatocellular carcinoma is increased,which may be involved in tumor proliferation and invasion.The high expression of CDC20 indicates poor prognosis of patients,and it is expected to become a tumor marker for judging the prognosis of hepatocellular carcinoma.