寄主转换对云斑白条天牛幼虫肠道细菌多样性的影响

Effects of Host Switching on Diversity of Intestinal Bacteria in Batocera lineolata Larvae

【目的】研究寄主转换对云斑白条天牛Batocera lineolata幼虫肠道细菌多样性的影响,为从肠道细菌的角度解析云斑白条天牛的寄主适应性以及开发利用微生物等方面提供理论依据。【方法】以核桃作为原寄主,桉树作为转换寄主饲养云斑白条天牛幼虫,采用传统微生物分离培养、16Sr RNA基因测序以及高通量测序的方法对转换寄主云斑白条天牛幼虫肠道细菌多样性进行研究。【结果】通过传统微生物培养共获得19株可培养细菌,从核桃饲养的云斑白条天牛幼虫肠道中分离出10株细菌,转换寄主后,从云斑白条天牛幼虫肠道中分离出9株细菌;两种寄主饲养处理均分离出6个属的可培养细菌,其中有5个属为共有菌属。高通量测序结果表明,寄主转换前后的云斑白条天牛幼虫肠道细菌多样性无显著差异。云斑白条天牛幼虫肠道样本中细菌分别属于20个门32个纲55个目90个科170个属,其中两种处理云斑白条天牛幼虫肠道优势菌均属于变形菌门(Proteobacteria)γ-变形菌纲(Gammaproteobacteria)肠杆菌目(Enterobacteriales)肠杆菌科(Enterobacteriaceae)肠杆菌科下未确定属(Uncultured bacterium f Enterobacteriaceae)【结论】两种处理云斑白条天牛幼虫肠道细菌种类组成相似,肠道细菌群落整体多样性比较丰富且菌群较为稳定,短期内改变寄主很难使其肠道细菌多样性发生改变。

【Objective】The study was carried out to investigate the effect of host switching on the diversity of intestinal bacteria in Batocera lineolata larvae,and find out the host adaptability of B.lineolata larvae from the perspective of intestinal bacteria,with a view to providing a theoretical basis for the development and utilization of microbes.【Method】B.lineolata larvae were raised with walnut as primary hosts and Eucalyptus as switching hosts,and the diversity of intestinal bacteria in B.lineolata larvae was studied by using the methods of traditional microbial culture,16Sr RNA gene sequencing and high-throughput sequencing.【Result】A total of 19 culturable strains of bacteria were obtained through traditional microbial culture.10 strains of bacteria were isolated from the intestine of the larvae of B.lineolata reared on the original host,and 9 strains were isolated from the larvae of B.reared by host;6 genera of culturable bacteria were isolated from both treatments,5 of which were common genera.High-throughput sequencing results showed that there were no significant differences in the diversity of intestinal bacteria in the larvae before and after host switching.B.lineolata larvae intestinal bacteria samples respectively belonged to 20 phylums,32 classes,55 orders,90 families and 170 genera,including,under two treatments,both B.lineolata larvae gut dominant fungi belonging to the Proteobacteri,Gammaproteobacteria,Enterobacteriales,Enterobacteriaceae and Uncultured bacterium f Enterobacteriaceae.【Conclusion】The composition of intestinal bacteria species of B.lineolata larvae under two treatments was similar,the overall diversity of intestinal bacterial community was relatively rich and stable,and it was difficult to change the diversity of intestinal bacteria by changing the host in a short period of time.