沉默信息调节因子2同系物1在人瘢痕疙瘩组织中的表达及其与氧化应激指标相关性研究

Expression of silent information regulator 2 homologue 1 in human keloid tissue and its correlation with oxidative stress indicators

目的:研究沉默信息调节因子2同系物1(SIRT1)在人瘢痕疙瘩组织中的表达情况,并探讨其表达与氧化应激指标的相关性。方法:选取瘢痕疙瘩患者82例,根据病程不同分为A组(0-6个月)、B组(6-12个月)和C组(>12个月)。将瘢痕组织分为浸润部、增生部和老化部三部分。在正常组织和瘢痕疙瘩组织,采用实时荧光定量PCR(qPCR)法检测SIRT1 mRNA表达,采用免疫印迹法检测SIRT1、p65和p-p65蛋白表达,并检测氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)和晚期氧化蛋白产物(AOPP)]含量。结果:在各组内,SIRT1 mRNA和蛋白在瘢痕疙瘩组织中的表达水平显著低于正常组织(均P<0.05)。三组间SIRT1 mRNA和蛋白在正常组织和瘢痕疙瘩组织中的表达水平比较差异无统计学意义(均P>0.05)。在各组内,瘢痕疙瘩组织老化部、增生部SIRT1 mRNA和蛋白表达水平显著高于浸润部,且老化部高于增生部(均P<0.05)。三组间瘢痕疙瘩组织不同部位SIRT1 mRNA和蛋白表达水平比较差异无统计学意义(均P>0.05)。正常组织和瘢痕疙瘩组织p65蛋白表达水平比较差异无统计学意义(P>0.05)。瘢痕疙瘩组织p-p65蛋白表达水平和p-p65/p65,以及SOD、MDA和AOPP含量显著高于正常组织(均P<0.05)。在82例瘢痕疙瘩组织中,SIRT1 mRNA和蛋白表达水平与SOD、MDA和AOPP呈负相关(均P<0.05)。结论:SIRT1在人瘢痕疙瘩组织中表达降低,其表达水平与人瘢痕疙瘩病程无关,而与其在瘢痕疙瘩组织中的表达部位有关,并且与瘢痕疙瘩组织中氧化应激呈负相关。

Objective:To study the expression of silent information regulator 2 homologue 1(SIRT1)in human keloid tissue,and to explore the correlation between its expression and oxidative stress indicators.Methods:A total of 82 patients with keloids were selected and divided into group A(0-6 months),group B(6-12 months)and group C(>12 months)according to the course of the disease,and divided the scar tissue into three parts:infiltration part,hyperplasia part and aging part.In normal tissue and keloid tissue,real-time quantitative PCR(qPCR)was used to detect the expression of SIRT1 mRNA,and Western blot was used to detect the expression of SIRT1,p65 and p-p65 proteins,and the contents of oxidative stress indicators(SOD,MDA and AOPP)were detected.Results:In each group,the expression levels of SIRT1 mRNA and protein in human keloid tissues were significantly lower than those in normal tissues(all P<0.05).There was no significant difference in the expression of SIRT1 mRNA and protein in normal tissues and keloid tissues among the three groups(all P>0.05).In each group,the expression levels of SIRT1 mRNA and protein in aging parts and hyperplasia parts of keloid tissues were significantly higher than those in infiltration parts,and the expression levels of SIRT1 mRNA and protein in aging parts were higher than those in hyperplasia parts(all P<0.05).There was no significant difference in SIRT1 mRNA and protein expression in different parts among the three groups(all P>0.05).There was no significant difference in p65 protein expression between normal tissues and keloid tissues(P>0.05).The level of p-p65 protein expression,p-p65/p65,as well as the levels of SOD,MDA and AOPP in keloid tissues were significantly higher than those in normal tissues(all P<0.05).In 82 keloid tissues,SIRT1 mRNA and protein expression levels were negatively correlated with SOD,MDA and AOPP(all P<0.05).Conclusion:The expression level of SIRT1 in human keloid tissue is decreased,and its expression level is not related to the course of human keloid,but related to its expression site in keloid tissue,and negatively related to oxidative stress in keloid tissue.