磷脂酶D特性及其在磷脂酰丝氨酸合成中的应用

Characteristics of Phospholipase D and Application in the Synthesis of Phosphatidylserine

磷脂酰丝氨酸(Phosphatidylserine,PS)是一种新资源食品,在自然界中含量稀少,提取困难。磷脂酶D(Phospholipase D,PLD)是生物酶法制备磷脂酰丝氨酸的关键合成酶。本文主要介绍了PLD的分子结构特点、催化机理及酶活特性,并总结了目前PS的制备方法,重点对PLD酶催化制备磷脂酰丝氨酸的研究进展及反应体系进行了论述,并对催化制备PS反应体系的选择进行了分析和展望,以期为深化酶法技术制备PS的研究提供参考,以此推动新资源食品PS的工业化生产和应用。

Phosphatidylserine(PS) is a new resource food, which has less content in nature and the difficulty of extraction.Phospholipase D(PLD) is the critical synthetase in enzymatic preparation of PS. This paper mainly introduces the characteristics of molecular structure of PLD and the catalytic mechanism and enzyme activity, and summarizes the current preparation methods of PS, focusing on the research progress and reaction system of enzymatic synthesis, and analyzes and prospects the selection of the reaction system for PS preparation. It is expected to provide reference for deepening the research of enzymatic preparation of PS, so as to promote the industrial production and application of new resource food PS.