SOX3、SOX9在口腔鳞状细胞癌中的表达及其临床意义

Expression and clinical significance of SOX3 and SOX9 in oral squamous cell carcinoma

目的:探讨口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)和癌旁组织中SOX3、SOX9蛋白表达情况及临床意义。方法:采用免疫组织化学染色法检测60例OSCC组织及相应癌旁正常组织中SOX3、SOX9蛋白表达情况,分析SOX3、SOX9在OSCC中的表达及其与临床病理因素的相关性。结果:SOX3、SOX9在OSCC组织中阳性表达率均明显高于相应癌旁组织(P<0.01)。单因素分析显示,不同TNM分期和淋巴结转移情况的病人其SOX3、SOX9蛋白表达水平差异均有统计学意义(P<0.05~P<0.01),而不同性别、年龄、肿瘤分化程度病人的SOX3、SOX9表达水平差异均无统计学意义(P>0.05)。Logistic多因素回归分析显示,TNM分期、淋巴结转移均为SOX3、SOX9表达的独立影响因素(P<0.01)。相关分析显示,OSCC组织中SOX3与SOX9蛋白表达呈明显正相关关系(r=0.398,P<0.01)。结论:SOX3、SOX9可能参与OSCC的发生、发展过程,对肿瘤的侵袭和转移起促进作用。

Objective:To investigate the expression levels and clinical significance of SOX3 and SOX9 in the oral squamous cell carcinoma(OSCC).Methods:The expression levels of SOX3 and SOX9 protein in 60 cases of OSCC tissue and adjacent normal tissue were detected using immunohistochemistry method.The relationship between the expression levels SOX3 and SOX9,and clinicopathologic features were analyzed.Results:The positive rates of SOX3 and SOX9 in OSCC tissue were significantly higher than those in paracancerous normal tissue(P<0.01).The results of univariate analysis showed that the differences of the expression levels of SOX3 and SOX9 protein in patients with different TNM stages and lymph node metastasis were statistically significant(P<0.05 to P<0.01),while there was no statistical significance in the protein expression levels of SOX3 and SOX9 in patients with different gender,age and degree of tumor differentiation(P>0.05).The results of logistic multifactor regression analysis showed that the TNM stage and lymph node metastasis were the independent influencing factors of SOX3 and SOX9 expression(P<0.01).The results of correlation analysis showed that the expression of SOX3 was positively correlated with expression level of SOX9 protein in OSCC tissue(r=0.398,P<0.01).Conclusions:The SOX3 and SOX9 may be involved in the occurrence and development of oral squamous cell carcinoma,and promote the invasion and metastasis of tumor cells.