FQ-PCR联合ELISA在乙型肝炎检测中的应用价值

Application value of FQ-PCR combined with ELISA in detection of hepatitis B

目的探析实时荧光定量聚合酶链式反应(FQ-PCR)联合酶联免疫吸附试验(ELISA)在乙型肝炎检测中的临床价值。方法180例乙型肝炎患者,根据检测方法不同分为对照1组(59例)、对照2组(59例)及观察组(62例)。对照1组行FQ-PCR检测,对照2组行ELISA检测,观察组行FQ-PCR+ELISA检测。对比三组检测结果。结果观察组诊断乙型肝炎的准确率为96.77%,对照1组诊断乙型肝炎的准确率为84.75%,对照2组诊断乙型肝炎的准确率为81.36%。观察组诊断乙型肝炎的准确率明显高于对照1组与对照2组,差异具有统计学意义(P<0.05)。对照1组与对照2组诊断乙型肝炎的准确率比较,差异无统计学意义(P>0.05)。结论相对于单一检测手段,FQ-PCR联合ELISA在乙型肝炎检测时诊断准确率更高,可为临床治疗提供准确依据,具有临床应用价值。

Objective To discuss the clinical value of fluorescence quantitative polymerase chain reaction(FQ-PCR)combined with enzyme-linked immunosorbent assay(ELISA)in hepatitis B.Methods A total of 180 patients with hepatitis B were divided into control group 1(59 cases),control group 2(59 cases)and observation group(62 cases)according to different detection methods.Control group 1 underwent FQ-PCR detection,control group 2 underwent ELISA detection,and observation group underwent FQ-PCR+ELISA detection.The test results of the three groups were compared.Results The diagnostic accuracy of hepatitis B in the observation group was 96.77%,that in the control group 1 was 84.75%,and that in the control group 2 was 81.36%.The diagnostic accuracy of hepatitis B in the observation group was significantly higher than that in the control group 1 and the control group 2,and the difference was statistically significant(P<0.05).There was no statistically significant difference in the diagnostic accuracy of hepatitis B between control group 1 and control group 2(P>0.05).Conclusion Compared with single detection method,FQ-PCR combined with ELISA has higher diagnostic accuracy in hepatitis B,which can provide accurate basis for clinical treatment and has clinical application value.