何首乌多糖对氧自由基及抗氧化酶活性的作用研究

Effects of Polygonum multiflorum polysaccharides on oxygen free radicals and antioxidant enzyme activities

目的研究分析何首乌多糖对氧自由基及抗氧化酶活性的作用。方法40只小鼠,随机分为对照组、模型组、小剂量何首乌多糖组、大剂量何首乌多糖组,每组10只。对照组、模型组予以防腐剂-尼泊金乙酯灌胃,小剂量、大剂量何首乌多糖组分别予以50 mg/(kg·d)和150 mg/(kg·d)何首乌多糖灌胃。且模型组和小剂量、大剂量何首乌多糖组每日颈背部皮下注射5%D-半乳糖0.5 ml,对照组皮下注射等量生理盐水。比较四组小鼠血清、肝、肾组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量,肝、肾组织中的谷胱甘肽过氧化物酶(GSH-PX)活力,脑组织中脂褐质(LF)荧光值。结果模型组小鼠血清、肝、肾组织中SOD活力及肝、肾组织中的GSH-PX活力均较对照组显著下降,差异具有统计学意义(P<0.05);小剂量何首乌多糖组、大剂量何首乌多糖组小鼠血清、肝、肾组织中SOD活力及肝、肾组织中的GSH-PX活力均较模型组升高,且大剂量何首乌多糖组高于小剂量何首乌多糖组,差异具有统计学意义(P<0.05)。模型组小鼠血清、肝、肾组织中MDA含量均较对照组明显升高,差异具有统计学意义(P<0.05);小剂量何首乌多糖组、大剂量何首乌多糖组小鼠血清、肝、肾组织中MDA含量均较模型组降低,且大剂量何首乌多糖组低于小剂量何首乌多糖组,差异具有统计学意义(P<0.05)。对照组小鼠脑组织中LF荧光值为(18.04±1.65),模型组为(20.36±1.87),小剂量何首乌多糖组为(16.32±1.35),大剂量何首乌多糖组为(14.68±2.10)。模型组小鼠脑组织中LF荧光值较对照组升高,差异具有统计学意义(P<0.05);小剂量何首乌多糖组、大剂量何首乌多糖组小鼠脑组织中LF荧光值较模型组降低,且大剂量何首乌多糖组低于小剂量何首乌多糖组,差异具有统计学意义(P<0.05)。结论何首乌多糖可有效抑制脂质过氧化,清除氧自由基,提高内源性抗氧化酶活性。

Objective To study and analyze the effects of Polygonum multiflorum polysaccharides on oxygen free radicals and antioxidant enzyme activities.Methods A total of 40 mice were randomly divided into control group,model group,low-dose Polygonum multiflorum polysaccharide group and high-dose Polygonum multiflorum polysaccharide group,with 10 mice in each group.The control group and model group were treated with the preservative-ethylparaben by gavage,and the low-dose and high-dose Polygonum multiflorum polysaccharides groups were treated with 50 mg/(kg·d)and 150 mg/(kg·d)of Polygonum multiflorum polysaccharides by gavage.In addition,the model group and low-dose and high-dose Polygonum multiflorum polysaccharide groups were injected subcutaneously with 0.5 ml of 5%D-galactose in the back of the neck every day,and the control group was injected subcutaneously with the same amount of normal saline.The activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in serum,liver and kidney,the activity of glutathione peroxidase(GSH-Px)in liver and kidney and the fluorescence value of lipofuscin(LF)in brain were compared.Results The activities of SOD in serum,liver and kidney and GSH-Px in liver and kidney in the model group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).The activities of SOD in serum,liver and kidney and GSH-Px in liver and kidney of lowdose and high-dose Polygonum multiflorum polysaccharide groups were higher than those in model group,and the high-dose Polygonum multiflorum polysaccharide group was higher than low-dose Polygonum multiflorum polysaccharide group.All the differences were statistically significant(P<0.05).The content of MDA in serum,liver and kidney in the model group was significantly higher than that in the control group,and the difference was statistically significant(P<0.05).The content of MDA in serum,liver and kidney in low-dose and highdose Polygonum multiflorum polysaccharide groups were lower than those in model group,and the high-dose Polygonum multiflorum polysaccharide group was lower than low-dose Polygonum multiflorum polysaccharide group.All the differences were statistically significant(P<0.05).The fluorescence value of LF in the brain tissue of mice was(18.04±1.65)in the control group,(20.36±1.87)in the model group,(16.32±1.35)in the lowdose Polygonum multiflorum polysaccharide group,and(14.68±2.10)in the high-dose Polygonum multiflorum polysaccharide group(14.68±2.10).The fluorescence value of LF in the brain tissue of model group was higher than that of the control group,and the difference was statistically significant(P<0.05).The fluorescence value of LF in the brain tissue of low-dose and high-dose Polygonum multiflorum polysaccharide groups were lower than those in model group,and the high-dose Polygonum multiflorum polysaccharide group was lower than lowdose Polygonum multiflorum polysaccharide group.All the differences were statistically significant(P<0.05).Conclusion Polygonum multiflorum polysaccharides can effectively inhibit lipid peroxidation,scavenge oxygen free radicals and improve the activity of endogenous antioxidant enzymes.