姜瘟病生防菌幼套近明球囊霉N62的筛选鉴定

Screening and Identification of Biocontrol Strain Claroideoglomus etunicatum N62 against Ginger Wilt

姜瘟病是一种细菌土传毁灭性病害,目前尚无特别有效的防治药剂。丛枝菌根(Arbuscular mycorrhizal,AM)真菌在作物促生及防治病害方面极具潜力,为充分发掘姜瘟病的生防菌资源,本研究采用盆栽试验方法评价4株AM真菌对生姜的促生作用和对姜瘟病的防治效果,利用形态学和分子生物学相结合的方法对优良菌株进行分类鉴定。结果表明,接种AM真菌可有效促进生姜植株的生长及有效提高植株对姜瘟病的抗性,其中菌株N62处理的生姜植株表现的防治效果显著优于其他菌株,盆栽试验防效为58.6%;结合形态学和分子鉴定技术,将菌株N62鉴定为幼套近明球囊霉Claroideoglomus etunicatum。N62是一株具有开发利用价值的姜瘟病生防菌株。

Ginger wilt is a devastating disease caused by the soil-borne bacterial Ralstonia solanacearum. Till now, there is still no bactericides available for effective control of the disease in the field. Arbuscular mycorrhizal fungi have great potential in promoting crop growth and preventing diseases. In order to explore the biocontrol agents against ginger wilt, four Arbuscular mycorrhizal fungi strains were selected to evaluate the ginger growth promotion ability and control efficacy against the ginger wilt through pot culture method, and the dominant strain N62 was identified based on morphological characteristics and 18 S rDNA sequence analysis. All the four arbuscular mycorrhizal fungi strains had the ability of promoting ginger growth after inoculation. The control efficacy of N62 against the ginger wilt was found up to 58.6% in the pot culture method, significantly higher than that of other arbuscular mycorrhizal fungi strains. Morphological observation and 18 S rDNA sequence alignment analysis results showed that the N62 strain belonged to Claroideoglomus etunicatum. These results indicated that the strain N62 was of development and utilization value for biological control of ginger wilt disease.