冬凌草甲素对急性哮喘小鼠气道重塑的影响

Effects of Oridonin on Airway Remodeling in Asthmatic Mice

目的:探讨冬凌草甲素对急性哮喘小鼠气道重塑的影响及其可能的机制。方法:将清洁级雌性BALB/c小鼠随机分为4组,分别为正常组、模型组、冬凌草甲素10mg/kg干预组(低剂量干预组)、冬凌草甲素20mg/kg干预组(高剂量干预组)进行试验。建立OVA诱导的急性哮喘模型,造模成功后,低剂量干预组腹腔注射冬凌草甲素10mg/kg,高剂量干预组腹腔注射冬凌草甲素20mg/kg。正常组以PBS代替OVA进行致敏、生理盐水代替OVA激发。采用HE染色观察气道壁厚度;Masson染色观察气道周围胶原纤维沉积;免疫组化测定TGF-β1蛋白表达量,RT-PCR测定肺组织中TGF-β1mRNA表达水平。结果:与正常组比较,模型组及干预组的气道壁厚度、气道周围胶原纤维沉积、TGF-β1蛋白表达量均明显升高(P<0.05),差异具有统计学意义。与模型组比较,干预组的气道壁厚度、气道周围胶原纤维沉积、TGF-β表达量均明显降低(P<0.05),与低剂量冬凌草甲素组比较,高剂量冬凌草甲素干预组气道壁厚度、气道周围胶原纤维沉积、TGF-β1蛋白表达量均降低(P<0.05)。结论:冬凌草甲素能减轻哮喘小鼠气道重塑,可能是通过抑制TGF-β1表达来实现的。

Objective:To explore the possible mechanism of the effect of Rubescensine A on airway remodeling in mice with acute asthma.Methods:Clean-grade female BALB/c mice were randomly divided into 4 groups,namely normal group,model group,Rubescensine A 10mg/kg intervention group(low-dose intervention group),Rubescensine A 20mg/kg The intervention group(high-dose intervention group)was tested.An OVA-induced acute asthma model was established.After successful modeling,the low-dose intervention group was intraperitoneally injected with Rubescensine A 10 mg/kg,and the high-dose intervention group was intraperitoneally injected with Rubescensine A 20 mg/kg.The normal group was sensitized with PBS instead of OVA,and challenged with normal saline instead of OVA.The airway wall thickness was observed by HE staining;the deposition of collagen fibers around the airway was observed by Masson staining;the expression of TGF-β1 protein was determined by immunohistochemistry,and the expression level of TGF-β1 mRNA in lung tissue was determined by RT-PCR.Results:Compared with the normal group,the airway wall thickness,the deposition of collagen fibers around the airway,and the expression of TGF-β1 protein in the model group and the intervention group were significantly increased(P<0.05),and the difference was statistically significant.Compared with the model group,the airway wall thickness,the deposition of collagen fibers around the airway and the expression of TGF-βin the intervention group were significantly decreased(P<0.05).Compared with the low-dose oridonin A group,the airway wall thickness,the deposition of collagen fibers around the airway,and the expression of TGF-β1 protein in the high-dose oridonin A intervention group were all decreased(P<0.05).Conclusion:Rubescensine A can reduce airway remodeling in asthmatic mice,which may be achieved by inhibiting the expression of TGF-β1.