2%丁子香酚·香芹酚可溶液剂高效液相色谱分析

Analysis of 2%Eugenol·Carvacrol SL by HPLC

本研究建立了一种测定2%丁子香酚·香芹酚可溶液剂的高效液相色谱(HPLC)定量分析方法。采用HPLC法,使用ZORBAX SB-C_(18)色谱柱,以甲醇-0.1%磷酸水溶液(70∶30,V/V)作流动相,检测波长为275 nm,对2%丁子香酚·香芹酚可溶液剂中有效成分进行定量分析。结果表明,丁子香酚和香芹酚相关系数(R^(2))依次为0.9996和0.9997,均大于0.999;标准偏差分别为0.0049%和0.0040%,RSD分别为0.32%和0.75%,二者RSD均小于其RSDr;平均回收率分别高达99.52%和99.97%。该分析方法操作简便,分离效果好,线性关系好,精密度和准确度高,满足定性定量分析要求。

An analysis for the detection of 2%eugenol·carvacrol SL by HPLC was established.The 2%eugenol·carvacrol SL were separated and determined with ZORBAX SB-C_(18) column,methanol-0.1%phosphoric acid solution(70∶30,V/V)at 275 nm detection wavelength by HPLC method.The linear correlation coefficients of eugenol and carvacrol were 0.9996 and 0.9997(R^(2)>0.999),the standard deviations were 0.0049%and 0.0040%,and the RSD were 0.32%and 0.75%,respectively.Both of RSD were smaller than their RSDr.The average recoveries of eugenol and carvacrol were 99.52%and 99.97%,respectively.The method was an ideal mthod for the qualitative and quantitiative analysis of 2%eugenol·carvacrol SL with simple operation,good linearity,high precision and accuracy.