外泌体miRNA应用于甲状腺乳头状癌诊断的研究

Study on application of exosomal miRNA in diagnosis of papillary thyroid carcinoma

目的 观察外泌体miRNA在甲状腺乳头状癌(PTC)患者血清中的表达变化,进而评估其诊断效能。方法 以新疆军区总医院收治的6例PTC患者为PTC组,同期6例体检健康者为对照组,对血清标本分离收获的外泌体miRNA进行高通量测序,进而行生物信息学分析。结果 通过高通量测序分析,在PTC组血清外泌体中共检测到47个差异表达miRNA,其中miRNA-1289、miRNA-519b-5p、miRNA-122-3p等15个miRNA的差异表达倍数|log_(2)(FoldChange)|>2,与对照组差异有统计学意义(P<0.05),可能是诊断PTC的潜在生物标志物。进一步的GO分析和KEGG分析显示,差异miRNA的靶基因被聚集到30个信号路径,含MAPK、PI3K/Akt、Ras等与肿瘤发生、发展相关的信号通路。结论 在血清外泌体miRNA中探寻到一组有利于PTC诊断的生物标志物。

Objective To observe the expression change of exosomal miRNA in the serum of the patients with papillary thyroid carcinoma(PTC),and then to evaluate its diagnostic efficiency.Methods Six patients with PTC admitted and treated in the General Hospital of Xinjiang Military Region were selected as the PTC group,and contemporaneous 6 healthy subjects undergoing physical examination served as the control group.The exosomal miRNAs isolated and harvested from serum samples conducted the high-throughput sequencing.Then the bioinformatics analysis was performed.Results Through high-throughput sequencing analysis,a total of 47 differentially expressed miRNAs were detected in serum exosomes of the PTC group,in which 15 differential expression folds |log_(2)(FoldChange)|>2 such as miRNA-1289,miRNA-519b-5p and miRNA-122-3p,and the difference with the control group had statistical significance(P<0.05),which could be the potential biomarker for PTC diagnosis.Further GO analysis and KEGG analysis showed that the target genes of differential miRNAs were clustered into 30 signal pathways,including MAPK,PI3K/Akt,Ras and other signal pathways related to tumorigenesis and development.Conclusion In serum exosomal miRNA,a set of biomarkers conducive to the diagnosis of PTC are found.