莲房原花青素对AGEs诱导的小鼠肠道组织损伤和微生物紊乱的改善作用

Effect of Lotus Seedpod Oligomeric Procyanidins on Tissue Injuryand Intestinal Microbial Disturbance of Mice Induced by AGEs

研究莲房原花青素低聚体(LSOPC)对膳食晚期糖化终末产物(AGEs)诱导的小鼠肠道组织损伤和微生物紊乱的改善作用。选用健康的雄性小鼠为动物模型,分为4组,分别饲喂高AGEs AIN-93G饲料,高AGEs AIN-93G中分别添加质量分数为0.2%和0.5%LSOPC的饲料以及正常AIN-93G饲料(对照组),饲喂12周,分析AGEs对小鼠的体质量、进食及进水量、脏器指数、肠道组织损伤、结肠紧密连接蛋白和寡肽转运蛋白的表达、结肠内炎症因子及肠道菌群的影响以及LSOPC的改善作用。实验结果表明,0.2%LSOPC和0.5%LSOPC均对高AGEs诱导的小鼠组织损伤有明显的改善作用,同时也增加了结肠内紧密连接蛋白ZO-1、Occludin的表达量,减少寡肽转运蛋白Pept1的表达量,结肠内的炎症因子TNF-α、IL-6及黏附因子VCAM-1、ICAM-1的表达量,小鼠肠道环境和肠道微生物紊乱也得到较为明显的改善,且与添加LSOPC呈剂量依赖关系。结论:LSOPC能够减轻膳食AGEs引起的小鼠肠道组织损伤和肠道微生物紊乱,具有发展为基础膳食营养补充剂的潜力。

To study the effects of lotus seedpod oligomeric procyanidins(LSOPC)on tissue damage and intestinal microflora disorder in mice induced by advanced glycation end products(AGEs),healthy male mice as animal model were divided into 4 groups and fed 12 weeks with high AGEs AIN-93G,high AGEs AIN-93G added 0.2%or 0.5%LSOPC(w/w),and normal AIN-93G as the control group.The effects of AGEs and LSOPC on mice weight,diet,water intake,organs indexs,intestinal tissue damage,colonic tight junction proteins and oligopeptide transporter expression,intestinal inflammatory factor and intestinal microflora disorder were analysis.Our results showed that both 0.2%LSOPC and 0.5%LSOPC had obvious ameliorative effects on tissue injury,increased the expression of ZO-1 and occludin,and decreased the expression of Pept1,TNF-α,IL-6,VCAM-1 and ICAM-1.Intestinal environment and intestinal microbial disorder were also significantly improved,which were dose-dependent on LSOPC.Conclusion:LSOPC could reduce mice tissue damage and intestinal microflora disorder caused by dietary AGEs,and has the potential to develop into a basic dietary supplement.