复合虾青素增强免疫力功能评价

Evaluation of Compound Astaxanthin to Enhance Immunity Function

目的:评价复合虾青素增强免疫力的功能。方法:采用刀豆蛋白(ConA)诱导小鼠脾淋巴细胞转化试验、2,4-二硝基氟苯(DNFB)诱导小鼠迟发型耳肿(DTH)试验、抗体生成细胞数测定、血清溶血素生成试验、碳粒廓清试验和NK细胞活性测定试验,综合评价复合虾青素的增强免疫力功能。结果:与溶媒对照组相比,虾青素油、复合虾青素均可明显提升动物脾淋巴细胞增殖水平和小鼠迟发性耳肿水平,细胞免疫功能测定结果阳性,且复合虾青素活性优于虾青素油;与溶媒对照组相比,虾青素油、复合虾青素组溶血空斑数和血清抗体水平明显增加,体液免疫功能测定结果阳性,且复合虾青素活性优于虾青素油;与溶媒对照组相比,虾青素油、复合虾青素对小鼠碳粒廓清和NK细胞活性均无明显影响,单核-巨噬细胞功能、NK细胞活性测定结果阴性。结论:复合虾青素具有增强免疫力的功能。

Objective:To evaluate the immune function of compound astaxanthin.Methods:Splenic lymphocyte transformation test of mice induced by ConA,delayed ear swelling test of mice induced by DNFB,antibody generation cell count test,serum hemolysin generation test,carbon particle clearance test and NK cell activity test were used to comprehensively evaluate the immune-enhancing function of compound astaxanthin.Results:Compared with the solvent control group,astaxanthin oil and compound astaxanthin could significantly improve the proliferation level of splenic lymphocytes and delayed ear edema in mice,and the cellular immune function was positive,and the activity of compound astaxanthin was better than astaxanthin oil.The hemolytic plaque number and serum antibody level were significantly increased,the humoral immune function was positive,and the activity of compound astaxanthin was better than astaxanthin oil.There was no significant effect on carbon clearance and NK cell activity in mice,and the results of mononuclear macrophage function and NK cell activity were negative.Conclusion:The compound astaxanthin has the function of enhancing immunity.