circ_0003998靶向miR-1184调控食管癌细胞Eca109增殖、迁移及侵袭的机制研究

The molecular mechanism of circ_0003998 targeting miR-1184 to regulate the proliferation,migration and invasion of esophageal cancer cells Eca109 in vitro

目的探讨circ_0003998调控食管癌细胞Eca109增殖、迁移及侵袭的分子机制。方法qRT-PCR法检测食管癌组织与癌旁组织中circ_0003998、miR-1184的表达量;体外培养人食管癌细胞Eca109,si-NC、si-circ_0003998、miR-NC、miR-1184 mimics、si-circ_0003998与anti-miR-NC、si-circ_0003998与anti-miR-1184分别转染至Eca109细胞;CCK-8实验与克隆形成实验检测细胞增殖能力;Transwell实验检测细胞侵袭能力;划痕实验检测细胞迁移能力;双荧光素酶报告实验检测circ_0003998与miR-1184的靶向关系。结果食管癌组织中circ_0003998的表达量高于癌旁组织(P<0.05),miR-1184的表达量低于癌旁组织(P<0.05);转染si-circ_0003998或转染miR-1184 mimics后,细胞活力和划痕愈合率降低(P<0.05),细胞克隆形成数和侵袭细胞数减少(P<0.05);circ_0003998可靶向调控miR-1184的表达;共转染si-circ_0003998与anti-miR-1184后,细胞活力和划痕愈合率升高(P<0.05),细胞克隆形成数和侵袭细胞数增多(P<0.05)。结论干扰circ_0003998表达可通过靶向miR-1184而抑制食管癌细胞增殖、迁移及侵袭。

Objective To investigate the molecular mechanism of circ_0003998 targeting miR-1184 to regulate the proliferation,migration and invasion of esophageal cancer cells Eca109 in vitro.Methods The qRT-PCR was used to detect the expression levels of circ_0003998 and miR-1184 in esophageal cancer tissues and para-cancerous tissues.In vitro cultured human esophageal cancer cells Eca109,the si-NC,si-circ_0003998,miR-NC,miR-1184 mimics,si-circ_0003998 and anti-miR-NC,si-circ_0003998 and anti-miR-1184 were transfected into Eca109 cells,respectively.CCK-8 assay and clone formation assay were used to detect cell proliferation ability,and Transwell was used to detect cell invasion ability.Moreover the scratch test was used to detect cell migration ability,and the dual luciferase reporter experiment was used to detect the targeting correlation between circ_0003998 and miR-1184.Results The expression levels of circ_0003998 in esophageal cancer tissue were significantly higher than those in para-cancerous tissues(P<0.05),however,the expression levels of miR-1184 were significantly lower than those in that in para-cancerous tissues(P<0.05).After transfection with si-circ_0003998 or miR-1184 mimics,the cell viability,scratch healing rate,the number of cell clone formation and the number of invasive cells were significantly decreased(P<0.05).The circ_0003998 could target and regulate the expression of miR-1184.After co-transfection of si-circ_0003998 and anti-miR-1184,the cell viability,scratch healing rate,the number of cell clones and the number of invasive cells were significantly increased(P<0.05).Conclusion To interfere with the expression of circ_0003998 can inhibit the proliferation,migration and invasion of esophageal cancer cells by targeting miR-1184.