FISH 技术在生物冶金中的应用

Application of Fish Technology in Bio Metallurgy

微生物种类在生物冶金的过程中具有重要的作用,但是传统的培养方法已不能满足生物冶金研究的需要,随着现代科学技术的发展,荧光原位杂交(FISH)技术被越来越广泛的使用。FISH技术是一种不依赖于培养的微生物检测技术,具有高度安全性和敏捷性,主要用来检测矿中微生物的形态、数量和空间分布情况的一种现代分子生物学方法。该文主要介绍了荧光原位杂交(FISH)技术的原理、主要试剂、实验仪器、浸矿菌的固定方法和原位杂交过程,同时对FISH技术在生物冶金中的应用和前景进行了展望。

Microbial species play an important role in the process of bio metallurgy,but the traditional culture methods can not meet the needs of bio metallurgy research.With the development of modern science and technology,Fluorescence in Situ Hybridization(FISH)technology is more and more widely used.FISH technology is a microbial detection technology independent of culture,with high safety and agility.It is mainly used to detect the morphology,quantity and spatial distribution of microorganisms in mines.This paper mainly introduces the principle of FISH,main reagents,experimental instruments,fixation method of ore leaching bacteria and in situ hybridization process,and prospects the application and prospect of FISH technology in bio metallurgy.