振腹法对多囊卵巢综合征大鼠性激素水平及炎症因子肿瘤坏死因子-α、白介素-6的影响

The effect of abdominal vibration Tuina on sex hormone level and inflammatory factors TNF-α、IL-6 in rats with polycystic ovary syndrome(PCOS)

目的探讨振腹法对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠血清性激素水平与炎症因子的影响,为振腹法的临床应用推广提供科学依据。方法24只SD雌性大鼠随机分为空白组、模型组、二甲双胍组、振腹组,采用来曲唑溶液灌胃制备PCOS大鼠模型。模型制备成功后,二甲双胍组给予二甲双胍溶于1%羧甲基纤维素钠溶液灌服43 mg/(kg·d),连续给药15天;振腹组给予振腹法干预,15分钟/只,连续治疗15天。比较各组的大鼠卵巢形态结构的差异,酶联免疫吸附法检测性激素促黄体生成素、卵泡刺激素、雌二醇、睾酮及炎症因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin 6,IL-6)水平。结果与空白组相比,模型组卵巢形态呈多囊样改变,性激素促黄体生成素、卵泡刺激素、雌二醇、睾酮水平变化显著(P<0.05),炎症因子TNF-α、IL-6水平表达均高于空白组(P<0.05);与模型组相比,二甲双胍组、振腹组大鼠卵巢形态结构改变具有明显差异,血清促黄体生成素、卵泡刺激素、雌二醇、睾酮水平显著改变(P<0.05),炎症因子TNF-α、IL-6水平均降低(P<0.05);与二甲双胍组比较,振腹组大鼠卵巢形态结构、血清促黄体生成素、卵泡刺激素、雌二醇、睾酮水平存在显著差异(P<0.05)。结论振腹法可改善PCOS大鼠卵巢形态,良性调节PCOS大鼠血清性激素水平,降低PCOS大鼠外周炎症因子TNF-α、IL-6水平,这可能是振腹法治疗PCOS的机制之一。

Objective To explore the effects of abdominal vibration tuina on serum sex hormones and inflammatory factors in polycystic ovary syndrome(PCOS)rats,so as to provide scientific basis for its clinical application.Methods 24 female SD rats were randomly divided into the control group,the model group,the metformin group and the abdominal vibration group.The PCOS rat model was established by intragastric administration of letrozole solution.After the successful preparation of the model,the abdominal vibration group was treated with abdominal vibration intervention 15 min/day,continuous treatment for 15 days.The ovarian morphology and structure of rats in each group were compared.The levels of luteinizing hormone(LH),follicle stimulating hormone(FSH),estrogen(E2),testosterone(T)and inflammatory factors tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)were measured by ELISA.Results Compared with the control group,the ovarian morphology of the model group showed polycystic changes,it’s significantly increased of the levels of sex hormones LH,FSH,E2 and T in the model group(P<0.05).The expressions of inflammatory factors TNF-α and IL-6 in the model group were also significantly higher than that in the control group(P<0.05).Compared with the model group,the morphological and structural changes of the rats in the metformin group and the abdominal vibration group were significantly different,the levels of LH,FSH,E2 and T in the serum were significantly changed(P<0.05),and the levels of inflammatory factors TNF-α and IL-6 were decreased(P<0.05).Compared with the metformin group,there were significant differences in the morphology and structure of ovary,the levels of LH,FSH,E2 and T in the serum in the abdominal vibration group(P<0.05).Conclusion Abdominal vibration therapy can improve the ovarian morphology and ovarian index of rats,regulate the level of serum sex hormones in PCOS rats,and reduce the expression of peripheral inflammatory cytokines TNF-αand IL-6 in PCOS rats,which may be one of the mechanisms of abdominal vibrating therapy in the treatment of PCOS.