鱼鳔胶原蛋白-壳聚糖-海藻酸钠水凝胶促小鼠皮肤伤口愈合研究

Experiment of swim bladder collagen-chitosan-alginate hydrogel on skin wound healing in mice

以鳗鱼(Anguillidae)鱼鳔胶原蛋白为主药,采用Ca^(2+)交联法制备水凝胶敷料,利用扫描电镜观察其结构;通过在小鼠背部皮肤制作伤口试验模型,分别用0.9%生理盐水(对照组)和水凝胶敷料(试验组)进行护理,观察3、7、14 d时皮肤伤口的愈合情况,HE染色观察皮肤的组织学变化。结果表明,水凝胶在扫描电镜下呈现多孔隙结构,水凝胶组血清抗氧化能力高于对照组,炎症因子水平低于对照组。水凝胶敷料明显促进伤口愈合,减轻伤口红肿和炎症,皮肤修复完整疤痕较小。

The eel bladder collagen extracted by acetic acid was used as the main drug,combined with chitosan and sodium alginate.The hydrogel dressing was prepared by Ca^(2+) cross-linking method,and its structure was observed under the scanning electron micro⁃scope.Through the skin wound experimental in vivo,the control group was treated with 0.9%normal saline and the experimental group was treated with hydrogel dressing.Then,the healing of skin wound was observed at 3,7 and 14 days.The wound healing rate was cal⁃culated,the antioxidant and inflammatory indicators in serum were detected,and the histological changes of skin were observed by he⁃matoxylin-eosin staining.The results showed that hydrogel showed a polypore structure under the scanning electron microscope.The serum antioxidant capacity of the hydrogel group was higher than that of the control group,and the level of inflammatory factors was lower than that of control group.Hydrogel dressing can significantly promote wound healing,reduce wound redness and inflammation,and completely repair the skin with smaller scars.