CTRP3、TGF-β1在糖尿病肾病中的表达及相关性研究

Expression and correlation of CTRP3 and TGF-β1 in diabetic nephropathy

采用免疫组织化学法检测糖尿病肾病组(DN)及正常对照组(NC)大鼠肾组织CTRP3与转化生长因子β1(TGF-β1)的表达情况。采用病例对照,研究正常健康者(NC组)、单纯2型糖尿病(T2DM组)、早期糖尿病肾病(DN1组)和临床糖尿病肾病(DN2组)患者胰岛素代谢参数、血脂和肾功能等临床指标,ELISA法检测血清CTRP3和TGF-β1水平。结果表明:DN组大鼠肾组织TGF-β1表达增高(P<0.05),CTRP3低表达(P<0.05);ELISA检测显示DN2组血清CTRP3水平显著下降(P<0.01),而TGF-β1水平显著升高(P<0.01);DN2组HOMA-IR、HbA1c、TG、ACR、BUN、SCr、Cysc水平升高(P<0.05),而GFR水平降低(P<0.01);相关分析显示血清CTRP3与TGF-β1、HOMA-IR、FBG、HbA1c、SBP、TG、ACR、SCr、BUN、Cysc呈负相关(P<0.05),与GFR呈正相关(P<0.01);回归分析显示HOMA-IR、TGF-β1、ACR、GFR是CTRP3的影响因素。这一研究提示CTRP3、TGF-β1参与DN的发展过程,与胰岛素代谢参数、血脂和肾功能相关标志物有一定的相关性。

To explore the expression of CTRP3 and TGF-β1 in renal tissue of diabetic nephropathy rats(DN)and normal control rats(NC)by immunohistochemistry.Case-control study was conducted to observe the insulin metabolic parameters,blood lipid and renal function in healthy subjects(NC group),type 2 diabetes(T2 DM group),early diabetic nephropathy(DN1 group)and clinical diabetic nephropathy(DN2 group).The serum levels of CTRP3 and TGF-β1 were detected by ELISA.The results showed that the expression of TGF-β1 was increased in DN group(P<0.05),while the expression of CTRP3 was decreased(P<0.05).ELISA showed that the level of serum CTRP3 was significantly decreased(P<0.01)while TGF-β1 was significantly increased(P<0.01);The levels of HOMA-IR,HbA1 c,TG,ACR,BUN,SCr and Cysc in DN2 group were increased(P<0.05),while GFR were decreased(P<0.01).Correlation analysis showed that serum CTRP3 was negatively correlated with TGF-β1,HOMA-IR,FBG,HbA1 c,SBP,TG,ACR,SCr,BUN,Cysc(P<0.05),and positively correlated with GFR(P<0.01).Regression analysis showed that HOMA-IR,TGF-β1,ACR and GFR were the influencing factors of CTRP3.This study suggested that CTRP3 and TGF-β1 were involved in the development of DN,related to insulin metabolism parameters,blood lipid and renal function markers.