一种烟碱固体脂质纳米粒杀虫剂的制备与优化

Preparation and optimization of nicotine loaded solid lipid nanoparticles as insecticide

针对传统烟碱类杀虫剂易污染环境的问题,设计开发了一种处方材料、生产过程都相对环保的烟碱固体脂质纳米粒杀虫剂.实验结果表明:烟碱固体脂质纳米粒的最优处方为8.5%的氢化葵花籽油(脂质)、2.0%的聚乙烯醇61000(稳定剂)、1.5%的烟碱(主药)和加至100.0%的Na_(2)CO_(3)-NaHCO_(3)缓冲液(介质,pH9.0).制备烟碱固体脂质纳米粒的最佳工艺为在600bar下高压匀质法循环7次.在最优处方和最佳工艺下,制备得到的固体脂质纳米粒的平均动态光散射粒径(PCS)为452nm,其中50%颗粒的直径低于342nm,99%颗粒的直径低于850nm,多分散指数(PDI)为0.277,并且粒径在30d内无显著变化,包封率达到(12.3±4.2)%.因此,该烟碱固体脂质纳米粒杀虫剂可为研制环境友好型杀虫剂提供参考.

The traditional neonicotinoid insecticide could broke ecological balance,thus we designed nicotine loaded solid lipid nanoparticles(N-SLN)which contain environmental friendly ingredients and producing process.The results showed that the optimized formulation is 8.5%hydrogenated sunflower oil(HSO)as lipid phase,2.0%PVA 61000 as stabilizer,1.5%nicotine as active agent and up to 100.0%Na_(2)CO_(3)-NaHCO_(3) buffer(pH 9.0)as medium.The optimized preparation process of N-SLN is using high pressure homogenization at 600bar for 7 cycles.Under the optimized formulation and preparation process,the size characteristics of the produced N-SLN did not make a significant change within 30 days as following:the photon correlation spectroscopy(PCS)mean size was 452nm,the diameter analyzed by laser diffractometer was that more than 50%particles were smaller than 342nm,more than 99%particles were smaller than 850nm,and the polydispersity index(PDI)was 0.277.The encapsulation efficiency of nicotine was(12.3±4.2)%.The N-SLN fabricated in this study paves the way for more environmental friendly insecticide.