摘要
通过比较序列测定法,在ADAMTS1基因第7外显子发现C/G突变,建立了PCR-PvuⅡ-RFLP分型技术,并在淮猪新品系Ⅱ猪种中进行多态性分型,分析其基因型频率和等位基因频率,检测该位点的多态性并与产仔数性状进行关联分析。结果表明,在淮猪新品系Ⅱ猪群中,所有胎次产仔数呈现AB>BB>AA的趋势,AB型个体的所有胎次产仔数显著高于BB型个体0.665头(P<0.05)。因此,ADAMTS1基因在淮猪新品系Ⅱ猪群中与产仔数性状显著相关,可能为提高猪产仔数性状提供一个有用的分子标记。
By comparision of the sequences of ADAMTS1 gene,a C/G mutation in exon 7 was found and developed a PCR-PvuⅡ-RFLP genotyping assay.Genotype frequencyies and allele frequencies of this SNP were investigated in New Sstrain Huai PigⅡ.Association of the SNP and litter size was assessed in New Sstrain Huai PigⅡ.Statistical analysis demonstrated that it showed the tendency of AB>BB>AA in all parity.In addition,those with the AB genotype had additional 0.665 piglets born compared to the BB animals(P<0.05)in New Sstrain Huai PigⅡ.Therefore,ADAMTS1 gene has significant associated with litter size in New Sstrain Huai PigⅡ,could be a useful molecular marker in selection for increasing litter size in pigs.
作者
刘林清
李庆岗
王重龙
LIU Linqing;LI Qinggang;WANG Chonglong(Institute of Animal Husbandry and Veterinary Medicine,Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei 230031,China)
出处
《养猪》
2022年第3期70-72,共3页
Swine Production
基金
青年人才推荐创新基金(XMT2022-09)
安徽省生猪产业技术体系项目(AHCYTX-05-09)。
