耿马甘蔗白叶病植原体昆虫介体调查与检测

Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma

为探明云南蔗区甘蔗白叶病植原体的昆虫介体及优势种群,丰富甘蔗植原体病害相关理论和技术基础,制定适用于甘蔗白叶病的综合防控措施,2019年采用寻集法和扫网法对甘蔗白叶病发病最为严重的云南省临沧市耿马蔗区进行甘蔗白叶病植原体昆虫介体调查和巢式PCR检测分析。调查检测结果显示,采集到的大青叶蝉(Tettigoniella viridis)和条纹平冠沫蝉(Clovia conifer)2种昆虫介体均被检测为阳性,是甘蔗白叶病植原体的自然携带者,说明2种叶蝉可能为甘蔗白叶病植原体潜在昆虫介体;大青叶蝉若虫呈强阳性,初步确定为优势种群。鉴于目前云南蔗区甘蔗白叶病植原体传播方式主要是带毒蔗种和叶蝉类昆虫介体,建议建立甘蔗无病健康种苗繁育基地,及时杀灭蔗园中叶蝉类昆虫介体,从源头上控制甘蔗白叶病的扩散蔓延,降低田间自然传播速度。

To investigate the insect vector and dominant population of sugarcane white leaf(SCWL)phytoplasma in Yunnan can enrich the theoretical and technical basis of sugarcane phytoplasma disease control and provide reference for formulating comprehensive control measures for SCWL. The insect vectors of SCWL phytoplasma were investigated using visual search and sweep netting methods and detected by nested PCR in Gengma of Lincang City, Yunnan Province in 2019, where the occurrence of SCWL was the most serious. The investigation and detection results showed that two collected insect vectors Tettigoniella viridis and Clovia conifer were tested positive. They were natural carriers of SCWL phytoplasma, indicating that these two leafhopper species might be the potential insect vectors of SCWL phytoplasma. Meanwhile, the nymph of T.viridis was tested strongly positive and preliminarily identified as the dominant population. In view of the fact that the main transmission ways of SCWL phytoplasma currently are SCWL-infected seedcane and leafhopper insect vector, it is suggested to establish a sugarcane breeding base of disease-free and healthy seedcane and kill leafhopper insect vector in sugarcane planting area in time, so as to control the spread of SCWL from the source and reduce the natural transmission speed in field.