益生菌组合物对慢传输型便秘的改善作用

Alleviation of slow transit constipation by probiotics complex

探究益生菌组合物(鼠李糖乳杆菌LR-168、嗜酸乳杆菌LA-99和动物双歧杆菌BB-115)对慢传输型便秘的作用及机制。将50只6周龄雄性BABL/c小鼠随机分为空白组(生理盐水)、模型组(洛哌丁胺)、阳性对照组(洛哌丁胺+动物双歧杆菌BB-12:5×10^(8) CFU)、低剂量组(洛哌丁胺+益生菌组合物:5×10^(8) CFU)和高剂量组(洛哌丁胺+益生菌组合物:5×10^(9) CFU),灌胃21 d后处死,对小鼠生理生化指标进行测定。与模型组相比,益生菌组合物低、高剂量组显著提高小肠推进率、黑便粒数和黑便质量,显著降低首粒黑便时间;此外,灌菌处理后,血清和结肠组织的兴奋性神经递质(P物质、胃动素和胃泌素)分泌上升,抑制性神经递质(血管活性肽、生长抑素和内皮素-1)分泌下降;各组间的血清细胞因子水平无统计学差异;结肠组织的AQP3和c-kit基因转录量在益生菌干预后显著提高。益生菌组合物具有缓解慢传输型便秘的作用,这可能是通过影响胃肠调节肽分泌,以及AQP3和c-kit基因转录的水平来实现的。

As the popularity of probiotics,research on mechanisms of probiotics is becoming more and more in-depth.It is necessary to Figure out what and how these strains can do.Using loperamide-induced constipation mouse model,the effects and mechanisms of probiotics complex(Lactobacillus rhamnosus LR-168,Lactobacillus acidophilus LA-99 and Bifidobacterium animalis BB-115)were investigated.Fifty BABL/c male mice aged six-week-old were used to test alleviation effect of probiotics complex.Firstly,five groups were randomly divided:blank group(saline),model(loperamide),positive control(loperamide+Bifidobacterium animalis BB12:5×10^(8) CFU),low-dose group(loperamide and probiotics complex:5×10^(8) CFU)and high-dose group(loperamide and probiotics complex:5×10^(9) CFU).Extended defecation time of first black stool was determined as the criterion for success of constipation modeling.Then,the data of defecation performance such as small intestinal transit rat,number of black stools and weight of black stools were collected.Based on the enzyme-linked immunosorbent assay,gastrointestinal peptide neurotransmitters and cytokines were analyzed.In regard to the potential effect of constipation,which unbalanced levels of aquaporin 3(AQP3)and c-kit could bring out,quantitative real-time polymerase chain reaction was used to explore the mechanism behind,preliminarily.In the light of the criterion for the success of constipation modeling,it drew a conclusion that modeling of slow transit constipation mouse got success with loperamide induced.From the analysis of physiological changes,defecation performance was improved significantly in constipation mice after gavage with probiotics complex,specifically in small intestinal transit rate,number of black stools and weight of black stools.When the hypotheses about mechanisms of probiotics complex focused on the connection between gastrointestinal peptide and constipation,the secretion of excitatory neurotransmitters(substance P,gastrin and motilin)and inhibitory neurotransmitters(vasoactive intestinal peptide,somatostatin and endothelin 1)was observed.Outstanding variations were detected among model group and other groups,with the secretion of excitatory neurotransmitters increased and inhibitory neurotransmitters decreased,compared with model group.There was no obviously difference in cytokines reported in each group,but a slight decline in model group,elucidating a latent balance ability of probiotics.The transcription levels of AQP3 and c-kit,which could affect the transport of water and colonic peristalsis,were lifted notably after intervention with probiotics.With the intragastric administration of the probiotics complex,not only the defecation performance got improved,but also the levels of neurotransmitters and cytokines related to constipation were balanced.The probiotics complex could be taken as a preparation with great prospects for alleviating slow transit constipation.