基于代谢组学分析黑老虎果实营养成分及不同部位富集差异

Profiling of nutrient metabolites in Kadsura coccinea fruit and their enrichment patterns in pulp,peel,and seed tissues

近年来,中药材黑老虎的果实被作为一种新型水果而广受关注,为深入了解其营养价值,利用广泛靶向代谢组学技术检测代谢物,分析其中的营养成分及在果皮、果肉、种子的富集格局。结果显示,在黑老虎果实中鉴定出429个营养成分,其中果皮中有424个、果肉中429个、种子中391个,3个部位共有的为389个;氨基酸及其衍生物数量最多(占22.6%),其余主要为有机酸(18.6%)、游离脂肪酸(13.5%)、糖及醇类(12.8%)、核苷酸及其衍生物(11.2%)。果皮和种子营养物质丰度相当,但都明显低于果肉,其主要原因为有机酸、游离脂肪酸、溶血磷脂酰乙醇胺在果肉中显著向上表达。黑老虎果皮、果肉和种子有大量相同营养成分,可连同开发利用;3个部位都分别含有多种高度富集的营养成分,在食品和药用领域开发利用价值较大。

Recently,the fruit of Kadsura coccinea Lem,a plant used in traditional Chinese medicine,has attracted wide attention as a new type of fruit.To fully understand its nutritional value,widely-targeted metabolomics was used to identify the metabolites and analyze their enrichment patterns in the peel,pulp,and seed tissues.The results showed that 429 nutrient metabolites were identified in K.coccinea fruit.Among them,424 were found in the peel,429 in the pulp,391 in the seed,and 389 in all three tissues.The number of amino acids and their derivatives was the largest(22.6%),and the rest were mainly organic acids(18.6%),free fatty acids(13.5%),saccharides and alcohols(12.8%),and nucleotides and their derivatives(11.2%).Nutrients were equally accumulated in the peel and seed,but both were significantly lower than the pulp,which was mainly due to the significantly increased expressions of organic acids,free fatty acids,and lysophosphatidylethanolamine in the latter.Since the peel,pulp,and seed tissues of K.coccinea shared a large number of nutrient metabolites,they could be extracted and used together.All three tissues contain a variety of highly enriched nutrient metabolites,which are considered valuable in developing and utilizing foods and medicines.