基于UPLC-ESI-TOF-MS技术的姬松茸和双孢蘑菇酚化合物定性分析及体外抗氧化活性研究

Qualitative analysis of phenolic compounds from Agaricus brasiliensis and Agaricus bisporus by UPLC-ESI-TOF-MS and their antioxidant properties in vitro

采用超高效液相色谱-电喷雾飞行时间质谱对姬松茸和双孢蘑菇子实体中的酚化合物组成进行了初步分离鉴定,并对其体外抗氧化活性进行了分析。从2种子实体中共分离鉴定出16种酚化合物,分别为:甘氨酰-L-酪氨酸、β-N-(γ-谷氨酰)-4-甲酰基苯肼、甲基戊酸、5-羟基色氨酸、2-乙酰氨基-3-羟基苯甲酸、4-羟基苯甲醛、acromelic acid E、L-焦谷氨酰-L-亮氨酸、邻-丁二酰胺基-苯甲酸甲酯、L-焦谷氨酰-L-苯丙氨酸、5-羟脯氨酰-L-谷氨酸、3,4-二羟基苯甲酸、(2R,4R)-2-[(1H-吲哚-3-基)甲基]-4-氨基-2-羟基戊烷-1,5-二酸、环磷酰胺、阿朴鸟嘌呤A和犬尿氨酸。2种食用菌子实体的多酚含量分别为22.21和19.58 mg GAE/g,均具有较强的抗氧化活性,且姬松茸提取液的DPPH自由基清除能力、还原力和Fe^(2+)螯合能力均显著强于双孢蘑菇(P<0.05)。研究结果可为姬松茸和双孢蘑菇相关功能产品或添加剂开发提供一定的理论依据。

Phenolic compounds in fruit bodies of Agaricus brasiliensis and A.bisporus were identified by ultra performance liquid chromatography-electrospray time-of-flight-mass spectrometry(UPLC-ESI-TOF-MS).The antioxidant activities in vitro of the phenolic extracts were assessed.Totally 16 phenolic compounds were identified from the two fungi:N-glycyl-tyrosine,β-N-(γ-glutamyl)-4-formylphenylhydrazine,tyrosylglycylglycine,5-hydroxytryptophan,N-acetyl-3-hydroxyanthranilic acid,4-hydroxy-benzaldehyde,acromelic acid E,L-pyroglutamyl-L-leucine,methyl N-(3-carbamoylpropionyl)anthranilate,L-pyroglutamyl-L-phenylalanine,5-oxoprolyl-L-glutamic acid,3,4-dihydroxybenzoic acid,(2R,4R)-2-[(1H-indol-3-yl)methyl]-4-amino-2-hydroxypentane-1,5-dioic acid,cytoxazone,asponguanine A and kynurenine.The polyphenols contents in fruit bodies of A.brasiliensis and A.bisporus were 22.21 mg GAE/g and 19.58 mg GAE/g respectively.The results showed that both two mushrooms had strong antioxidant properties.The DPPH free radical scavenging ability,reducing power and Fe^(2+)chelating ability of A.brasiliensis were significantly stronger than that of A.bisporus(P<0.05).The results of this study could provide theoretical basis for the development of A.brasiliensis and A.bisporus as functional products or additives.