基于全长16S rRNA测序的血清高二胺氧化酶人群肠道菌群特征

Characterization of gut microbiota based on full-length 16S rRNA gene sequencing in high diamine oxidase population

目的探索肠道菌群与血清二胺氧化酶(diamine oxidase,DAO)水平之间的相关性,对DAO高水平(DAO-H)人群和正常人群的肠道菌群特征进行差异性分析。方法在战略支援部队特色医学中心2021年健康体检人群中招募62名成年志愿者,其中DAO-H人群31名,正常人群31名,采集粪便样本,通过使用16S rRNA全长基因测序技术,对所有受试者粪便样本进行分析,研究不同DAO水平人群的肠道菌群构成特征。结果DAO-H人群肠道菌群α多样性与正常人群差异无统计学意义,但是菌群结构和功能改变:共生细菌减少,如Phocaeicola、拟杆菌科细菌等;潜在致病菌增加,如肺炎克雷伯菌。DAO-H组的菌群代谢发生变化,菌群鞘脂代谢水平降低,万古霉素类抗生素生物合成(biosynthesis of vancomycin group antibiotics)、叶酸一碳库(one carbon pool by folate)、萜类骨架生物合成(terpenoid backbone biosynthesis)、细胞周期-Caulobacter(cell cycle-Caulobacter)、蛋白质输出(protein export)、碱基切除修复(base excision repair)、氮代谢(nitrogen metabolism)水平较正常组升高。结论血清中DAO-H人群与正常人群比较,存在特有的肠道菌群构成与菌群代谢特征。

Objective To investigate the correlation between gut microbiota and serum diamine oxidase(DAO)level and to analyze the differences in gut microbiota between high DAO(DAO-H)and normal DAO populations.Methods This study recruited 62 adult volunteers(31 in DAO-H group and 31 in normal control group)taking health examination in the Strategic Support Forces Special Medical Center in 2021.Their stool samples were collected to analyze the composition of gut microbiota in the two populations by full-length 16S rRNA gene sequencing.Results No significant difference in the alpha diversity of gut microbiota was found between the DAO-H group and the normal control group,but the structure and function of gut microbiota varied.In the DAO-H group,commensal bacteria decreased,such as Phocaeicola and Bacteroidetes,while potential pathogenic bacteria increased,such as Klebsiella pneumoniae.There were changes in the metabolism of gut microbiota in the DAO-H group,including inhibited sphingolipid metabolism and enhanced biosynthesis of vancomycin group antibiotics,one carbon pool by folate pathway,terpenoid backbone biosynthesis,cell cycle-Caulobacter,protein export,base excision repair and nitrogen metabolism.Conclusions Compared with the people with normal DAO,the population with high DAO had unique characteristics in gut microbiota composition and metabolism.