胃肠安丸挥发性成分分析及GC-MS指纹图谱建立

Analysis of volatile components and establishment of GC-MS fingerprints for Weichang’an Pills

目的分析胃肠安丸(木香、檀香、沉香等)挥发性成分,并建立其GC-MS指纹图谱。方法乙酸乙酯提取挥发性成分,GC-MS结合保留指数进行鉴定,聚类分析、主成分分析、正交偏最小二乘法判别分析进行化学模式识别。结果鉴定了102种挥发性成分,其中和厚朴酚、厚朴酚,三乙酸甘油酯,单硬脂酸甘油酯,2-单棕榈酸甘油相对含量高于5%。20批样品GC-MS指纹图谱中有35个共有峰,相似度均大于0.90。各批样品按生产日期被聚为2类,并筛选出12种主要差异成分。结论该方法稳定可靠,可用于胃肠安丸的质量控制。

AIM To analysis volatile components in Weichang’an Pills(Aucklandiae Radix,Santali albi Lignum,Aquilariae lignum Resinatum,etc.)and to establish their GC-MS fingerprints.METHODS The volatile components were extracted by ethyl acetate,after which the identification was performed by GC-MS combined with retention index,and cluster analysis,principal component analysis and orthogonal partial least square discriminant analysis were adopted in the chemical pattern recognition.RESULTS One hundred and two volatile components were identified,among which honokiol,magnolol,glycerol triacetate,glyceryl monostearate and 2-monopalmitin demonstrated the relative contents of more than 5%.There were thirty-five common peaks in the fingerprints of twenty batches of samples with the similarities of more than 0.90.Various batches of samples were clustered into two types according to production date,and twelve main differential constituents were screened.CONCLUSION This stable and reliable method can be used for the quality control of Weichang’an Pills.