摘要
为探明兽用鸡脾和猪脾转移因子(Transfer factor,TF)与免疫活性相关的多肽种类及其氨基酸组成,采用液相色谱-质谱联用法(LC-MS)对其进行多肽测序,并与蛋白质比对。结果发现,由鸡脾和猪脾制备的TF分别检测到免疫相关多肽45条和102条,多肽的氨基酸数量为5~24个,分子量为599.2784~2507.8258 Da;这些多肽对应于免疫活性蛋白质64种,以细胞免疫和先天免疫活性为主。该研究揭示了兽用鸡脾和猪脾TF的免疫活性多肽的组成及其氨基酸序列,有助于进一步研究其有效成分和作用机理。
To explore the peptide species and their amino acid composition of chicken-derived spleen and pig-derived spleen transfer factors(TF)associated with immune activity in veterinary,they were polypeptide sequenced using liquid chromatography-mass spectrometry usage(LC-MS)and aligned to proteins.The results confirmed that 45 and 102 peptides were detected in the chicken and pig spleen TF respectively,with the peptide of 5 to 24 amino acids and the molecular weight of 599.2784~2507.8258 Da.These peptides correspond to 64 immunoactive proteins,mainly possessing cellular and innate immune activity.This study revealed the composition and amino acid sequence of immunoactive peptides associated with the veterinary TF in chicken and pig spleen,which is important for studying its effective components and mechanism.
作者
黄迪海
盛晓丹
刘霞
黄兵
亓丽红
张再辉
秦卓明
HUANG Di-hai;SHENG Xiao-dan;LIU Xia;HUANG Bing;QI Li-hong;ZHANG Zai-hui;QIN Zhuo-ming(Shandong Jianmu Biological Pharmaceutical Co., Ltd., Jinan 250100, China;Jinan Center of Engineering Technology Research for Animal Biologics, Jinan 250100, China;Institute of Poultry Science Shandong Academy of Agricultural Science, Jinan 250023, China)
出处
《中国兽药杂志》
2022年第5期10-19,共10页
Chinese Journal of Veterinary Drug
基金
山东省农业科学院农业科技创新工程(CXGC2022A25)
山东省科技成果转移转化补助(鲁渝科技协作)项目“家禽养殖替抗饲料添加剂的应用与示范”。
关键词
转移因子
多肽
氨基酸序列
免疫激活
transfer factor
peptides
amino acid sequences
immunoactive
