高效液相色谱-串联质谱法测定猪肉中氯霉素残留量

Determination of Chloramphenicol Residues in Pork by High Performance Liquid Chromatography Tandem Mass Spectrometry

本文建立了高效液相色谱-串联质谱法测定动物源性食品猪肉中氯霉素残留量的分析方法。试样采用乙腈提取,LC-Si硅胶小柱净化,液相色谱质谱联用仪(Liquid Chromatography Tandem Mass Spectrometry,LC-MS/MS)使用电喷雾负离子扫描(ESI-),多反应监测(Multiple Reaction Monitoring,MRM)模式检测,以氯霉素-D_(5)为内标定量。结果表明,氯霉素在0.5~100.0 ng/mL线性关系良好,检出限与定量限分别为0.04 μg/kg、0.1 μg/kg,加标回收率为80.4%~100.0%,精密度为1.06%~8.33%,该方法适用于猪肉等动物源性食品中氯霉素残留量的检测。

A method for the determination of chloramphenicol residues in animal derived food pork by high performance liquid chromatography tandem mass spectrometry was established.The samples were extracted with acetonitrile,purified by LC-Si silica gel column,electrospray negative ion scanning (ESI-) with liquid chromatography tandem mass spectrometry (lc-ms/ms),detected in multiple reaction monitoring (MRM) mode,and quantified with chloramphenicol-D_(5)as the internal standard.The results showed that the linearity of chloramphenicol was good in the range of 0.5~100.0 ng/mL,the detection limit and quantitative limit were 0.04 μg/kg and 0.1 μg/kg respectively,the recovery was in the range of 80.4%~100.0%,and the precision was 1.06%~8.33%,the method is suitable for the determination of chloramphenicol residues in animal derived foods such as pork.