大豆GmCBL7-2生物信息学及非生物胁迫表达分析

Bioinformatics and abiotic stress expression analysis of GmCBL7-2 in soybean

钙调磷酸酶B类似蛋白(CBL)作为植物钙离子信号感受器,在植物非生物胁迫和信号转导中发挥重要作用。对大豆GmCBL7-2基因进行生物信息学分析及非生物胁迫下表达量的检测。GmCBL7-2基因开放阅读框全长639 bp,编码一个含212个氨基酸的蛋白质,相对分子量24.4 kD,等电点4.56,属于亲水性蛋白。预测结果显示,GmCBL7-2蛋白主要定位于细胞质中,含有13个磷酸化位点。GmCBL7-2蛋白质3D结构模型中存在两个Ca2+结合位点。GmCBL7-2蛋白与拟南芥AtCBL7蛋白亲缘关系最近。表达量检测结果显示,GmCBL7-2在大豆幼苗中可以响应干旱、高盐及低温胁迫,且对干旱胁迫的响应更加显著。

Calcineurin B-like protein(CBL)act as calcium ion signaling receptor,which play an important role in plant abiotic stress and signal transduction.In this study,the GmCBL7-2 gene from soybean was analyzed by bioinformatics and its expression level was detected under abiotic stress.The open reading frame of GmCBL7-2 was 639 bp,encoding a protein containing 212 amino acids with relative molecular weight of 24.4 kD and isoelectric point of 4.56.GmCBL7-2 protein was a hydrophilic protein.The predicted results showed that GmCBL7-2 protein was mainly located in the cytoplasm and contained 13 phosphorylation sites.There were two Ca2+binding sites in 3D structural model of GmCBL7-2 protein.GmCBL7-2 protein is most closely related to Arabidopsis AtCBL7 protein.The expression level of GmCBL7-2 in soybean seedlings showed that GmCBL7-2 could respond to drought,salt and cold stresses,and the response to drought stress was more significant.