高通量测序技术在害虫和害螨抗药性研究中的应用

Application of high-throughput sequencing in the study of acaricide resistance

螨类是陆生动物中仅次于昆虫的第2大类群。长期以来,对害螨抗药性的研究都落后于害虫。近年来,随着高通量测序技术的出现,开辟了害虫和害螨抗性机理研究和抗性监测的新局面。本文概述了高通量测序技术应用于害虫和害螨抗药性机理研究已取得的进展,介绍了利用高通量测序技术开发的抗药性监测新方法,并提出了害虫和害螨抗药性机理研究和田间种群抗性水平监测的发展方向。

Mites are the second-largest group of terrestrial animals following insects.The research in acaricide resistance of mites,however,has been substantially lagged behind.In recent years,the advent of genomics era has afforded researchers with high-throughput sequencing technologies to address outstanding biological questions.In this review,we summarized the current progresses in mechanistic studies in acaricide resistance of pest insects and mites and resistance monitoring using high-throughput sequencing approaches.Finally,we concluded this review by sharing our perspectives on future research avenues.