CRISPR-dCas系统在食品微生物转录调控与多酶级联中的应用及展望

Application and Prospect of CRISPR-dCas System in Transcriptional Regulation of Food Microorganisms and Multi-Enzymes Cascading

基于成簇规律间隔短回文重复序列(cluste redregularly interspacedshort palindromic repeats,CRISPR)及其关联Cas蛋白构成的CRISPR-Cas系统已经成为一种革命性基因组编辑工具.近年来,由此衍生而来的CRISPR-dCas(核酸酶活性失活Cas蛋白)系统也被开发成多种高效工具应用于微生物研究领域.本文综述了CRISPR-dCas系统在食品微生物领域,尤其是基于dCas9和dCpf1蛋白的CRISPR系统在食品酿造微生物领域(如酿酒酵母Saccharomyces cerevisiae、枯草芽孢杆菌Bacillus subtilis和谷氨酸棒杆菌Corynebacterium glutamicum等)中的转录调控以及多酶级联方向的研究进展,为CRISPR-dCas系统在食品微生物中的应用研究提供参考思路.

The CRISPR-Cas system that bases on the clustered regularly interspaced short palindromic repeats(CRISPR)and their associated Cas proteins have become a revolutionary tool in the genome editing.In recent years.the derived CRISPR-dCas(nuclease deactivated Cas protein)system is developed into a variety of eficient tols for microbial applications.In this article.the appication of CRISPR dCas system in food microbiology has been reviewed.espeilly the transcriptional regulation based on dCas9 and dCpf1 in fond fermentation microorganisms.such as Saccharamyces cerevisiae.Bacillus sbhtilis.and Corymebacterium glutamicsm.as well as the application in multi-enzymes cascading.This review expects to provide references and help for the research in food microorganisms using CRISPR-dCas system.