脂联素介导SIRT1/PGC-1α信号通路对大鼠脑缺血损伤后的影响

Effects of adiponectin-mediated SIRT1/PGC1-αsignaling pathway on cerebral ischemic injury in rats

目的研究脂联素(APN)介导SIRT1/PGC-1α信号通路对大鼠脑缺血损伤后的影响。方法2019年10月—2020年12月于武汉科技大学动物实验中心进行实验。SPF级雄性SD大鼠60只,随机数字表法分为空白对照组、模型组、脂联素组、Sirtonol(SIRT1抑制剂)组、脂联素+Sirtonol组,每组各12只,除空白对照组外,均建立脑缺血损伤模型,并进行相应药物干预。造模24 h后取大鼠脑组织,HE染色观察大鼠脑组织的病理损伤程度,ELISA法检测大鼠脑组织氧化因子ROS、MDA及SOD水平,Western-blot法检测SIRT1及PGC-1α蛋白表达水平。结果与空白对照组比较,模型组大鼠脑组织损害程度加重,氧化因子ROS、SOD水平显著升高,MDA水平显著降低,差异均有统计学意义(t/P=13.737/<0.001、13.509/<0.001、15.552/<0.001),SIRT1及PGC-1α蛋白表达均显著减少(t/P=22.764/<0.001、28.645/<0.001);与模型组比较,脂联素组大鼠脑组织损伤程度减轻,氧化因子ROS、SOD水平降低,MDA水平升高(t/P=12.225/<0.001、14.019/<0.001、14.067/<0.001),SIRT1及PGC-1α的蛋白表达增加(t/P=20.089/<0.001、27.937/<0.001),Sirtonol组氧化因子ROS、SOD水平升高,MDA水平降低(t/P=18.508/<0.001、17.836/<0.001、16.769/<0.001);与脂联素组比较,脂联素+Sirtonol组氧化因子ROS、SOD水平升高,MDA水平降低(t/P=15.079/<0.001、18.470/<0.001、19.067/<0.001)。结论脂联素介导的SIRT1/PGC-1α信号通路可以减轻对大鼠的脑缺血损伤。

Objective To study the effect of adiponectin(APN)-mediated SIRT1/PGC1-αsignaling pathway on cerebral ischemic injury in rats.Methods From October 2019 to December 2020,experiments were conducted at the Animal Experiment Center of Wuhan University of Science and Technology.Sixty SPF male SD rats were randomly divided into blank control group,model group,adiponectin group,Sirtonol(SIRT1 inhibitor)group,and adiponectin+Sirtonol group,with 12 rats in each group,except for the blank group.Except for the control group,a model of cerebral ischemia injury was established,and corresponding drug intervention was carried out.24 h after modeling,the rat brain tissue was collected,HE staining was used to observe the degree of pathological damage in the rat brain tissue,ELISA method was used to detect the levels of oxidative factors ROS,MDA and SOD in the rat brain tissue,and Western blot method was used to detect the protein expression levels of SIRT1 and PGC1-α.Results Compared with the blank control group,the damage degree of brain tissue in the model group was aggravated;the levels of oxidative factors ROS and SOD increased,and the level of MDA decreased(t/P=13.737/0.000,13.509/0.000,15.552/0.000);SIRT1 and PGC1-αproteins expression decreased(t/P=22.764/0.000,28.645/0.000).Compared with the model group,the adiponectin group reduced the degree of brain tissue damage;the levels of oxidative factors ROS and SOD decreased;the levels of MDA increased(t/P=12.225/0.000,14.019/0.000,14.067/0.000);SIRT1 and PGC1-αThe protein expression increased in the Sirtonol group(t/P=20.089/0.000,27.937/0.000);the levels of oxidative factors ROS and SOD were increased in the Sirtonol group;Compared with the adiponectin group,the levels of oxidative factors ROS and SOD in the adiponectin+Sirtonol group were increased,and the levels of MDA were decreased(t/P=15.079/0.000,18.470/0.000,19.067/0.000).Conclusion Adiponectin-mediated SIRT1/PGC1-αsignaling pathway can alleviate cerebral ischemia injury in rats.