贝莱斯芽孢杆菌JTB8-2对加工番茄促生作用及其安全性评价

Growth Promoting Effect and Safety Evaluation of Bacillus velezensis JTB8-2 on Processed Tomato

【目的】研究新疆加工番茄田土壤中获得的贝莱斯芽孢杆菌JTB8-2对加工番茄促生作用和安全性。【方法】采用种子催芽、穴盘育苗和盆栽试验方法。【结果】贝莱斯芽孢杆菌JTB8-2菌株发酵菌液25、50和100倍稀释液处理的加工番茄植株株高、茎粗、茎鲜重和根鲜重均显著高于空白对照;菌株JTB8-2发酵液25倍液、50倍液和100倍液处理的番茄植株茎鲜重显著高于NB培养基25倍液、50倍液、100倍液和空白对照;菌株JTB8-2菌株发酵液原液对20种作物种子萌发和芽生长具有抑制作用,25倍及以上稀释液对作物种子萌发和芽生长无影响。【结论】贝莱斯芽孢杆菌JTB8-2菌株发酵液能够促进番茄植株生长,其发酵液原液不宜做作物浸种处理,发酵液25倍及以上稀释液可以浸种。

【Objective】This project aims to make clear the growth-promoting effects and security of B.velezensis JTB8-2 obtained from local processed tomato fields in Xinjiang.【Method】The study was carried out by seed germination promotion,hole tray seedling and pot cultivation.【Results】The seedling test results showed that the plant height,stem diameter,stem fresh weight and root fresh weight of processed tomato treated with 25,50 and 100 times dilution of fermentation broth of B.velezensis JTB8-2 were significantly higher than those of blank control.The results of pot experiment showed that the fresh weight of tomato stem treated by fermentation solution 25 times,50 times and 100 times was significantly higher than those treated by NB medium 25 times,50 times and 100 times and blank control.The results of seed germination test showed that the original fermentation liquid of strain JTB8-2 had inhibitory effect on seed germination and bud growth of 20 crops,but the dilution liquid of 25 times or more had no effect on seed germination and bud growth of crops.【Conclusion】It is safe for seed germination and bud growth of crops to dilute fermentation broth 25 times or more,and the fermentation broth of B.velezensis JTB8-2 can promote the growth of tomato plants.The strain has the potential to be developed into a growth-promoting bacterium of processed tomato and more crops.