基于宏基因组学的亚硝酸盐氧化菌属的新陈代谢功能分析

Metabolic function analysis of nitrite oxidizing bacteria based on metagenomics

为探究亚硝酸盐氧化菌(NOB)的菌群结构及其新陈代谢功能特性,在富集NOB菌属的基础上,基于宏基因组学技术探究了富集活性污泥系统内NOB微生物种群结构、相互作用关系、氮转化相关功能基因及蛋白质功能特性。结果表明,Nitrobacter为富集后活性污泥系统中最优势菌属,相对丰度为37.8%。微生物菌种之间表现出各种错综复杂的关联特征,其中,维氏硝酸杆菌种(Nitrobacter winogradskyi)和汉氏硝化菌种(Nitrobacter hamburgensis)呈现出显著正相关性。此外,新陈代谢是系统内各类菌属中主导的生命活动过程,亚硝酸盐氧化还原酶Nxr为生化系统内氮代谢的关键酶(占比13.5%)。本研究结果可为NOB菌属的富集培养提供参考。

In order to explore the community structure and metabolic function of nitrite oxidizing bacteria(NOB) in an activated sludge system, the microbial population structure, interaction relationship, nitrogen conversion related functional factors and protein functional characteristics in the NOB enriched activated sludge system were explored based on metagenomics technology. Results showed that Nitrobacter was the most abundant bacteria in the activated sludge system after enrichment, with relative abundance of 37.8%. Among them, Nitrobacter winogradskyi and Nitrobacter hamburgensis showed a strong positive correlation. In addition,metabolism was the most dominant process of all kinds of bacteria in the system, and Nitrite oxidoreductase Nxr was the key enzyme of nitrogen metabolism in biochemical system(accounting for 13.5%). The results of this study can provide a reference for the enrichment culture of NOB bacteria.