食品加工机械材料橡胶密封垫圈中5种荧光增白剂迁移量的测定及其迁移规律研究

Investigation on Determination of Migration of Five Fluorescent Whit⁃ening Agents in Food Processing Machinery Material Sealing Rubber Washer and Their Migration Law

建立了一种高效液相色谱-串联质谱同时测定食品加工机械材料橡胶密封垫圈中5种荧光增白剂(FWA184、FWA185、FWA393、FWA367、FWA135)在不同食品模拟物中迁移量的检测方法。研究了食品加工机械材料橡胶密封垫圈中5种荧光增白剂在水、4%乙酸水溶液、50%乙醇水溶液和橄榄油4种食品模拟物中的迁移规律。实验结果表明,5种荧光增白剂在5~100μg/L范围内线性良好,相关系数r≥0.9991,方法定量下限为0.001~0.050 mg/kg。在橄榄油基质中,加标水平为0.05、0.1、0.5 mg/kg时,回收率为81.8%~101%,相对标准偏差为1.2%~5.6%(n=10)。方法快速简便,灵敏度、回收率和准确性能够满足食品接触材料及制品中荧光增白剂迁移量的测定要求。考察了食品模拟物种类、迁移温度、迁移时间对迁移量的影响。结果表明,在相同条件下,橡胶密封垫圈中5种荧光增白剂在橄榄油模拟物中迁移量最高;当温度升高时迁移量显著增加;随着时间的延长,迁移量呈现由快速迁移至缓慢迁移,随后趋于平衡的过程。

An high performace liquid chromatography-tandem mass spectrometric method was established for determination of the specific migration of five fluorescent whitening agents,i.e FWA184,FWA185,FWA393,FWA367 and FWA135 in food processing machinery material sealing rubber washer.The migration law for 5 fluorescent whitening agents transferring from food processing machinery material sealing rubber washer into 4 food simulants(water,4%acetic acid,50%ethanol and olive oil)was investigated.The results showed that there were good linear relationships for five fluorescent whitening agents in the concentration range of 5-100μg/L,with their correlation coefficients(r)not less than 0.9991.The limits of quantitation(LOQ)of the method were 0.001-0.050 mg/kg.In olive oil,the average recoveries at three spiked levels of 0.05,0.1,0.5 mg/kg ranged from 81.8%-101%,with relative standard deviations(RSDs,n=10)of 1.2%-5.6%.As proved,this method was simple and fast,which satisfies the requirements(including sensitivity,specificity and selectivity)for determining fluorescent whitening agents in food processing machinery material sealing rubber washer.Under the same conditions,the migration of 5 fluorescent whitening agents in sealing rubber washer was the highest in olive oil.With the increase of temperature,the migration of 5 fluorescent whitening agents increased significantly,while with the extension of contact time,the migration of 5 fluorescent whitening agents showed a process from rapid migration to slow migration,and then tended to reach an equilibrium.