金银花不同发育时期挥发性成分的HS-SPME-GC-MS分析

Analyses of volatile components in Lonicerae Japonicae Flos at different growth stages by HS-SPME-GC-MS

目的探讨金银花Lonicerae Japonicae Flos不同发育时期挥发性成分的差异。方法采用顶空固相微萃取-气质联用技术(HS-SPME-GC-MS),结合保留指数方法,对米蕾期(RB)、三青期(TG)、二白期(TW)、大白期(BW)、银花期(HS)、金花期(GF)6个不同时期金银花的挥发性成分进行定性定量分析,并通过主成分分析和聚类分析对6个不同生长时期的金银花挥发性物质进行判定、区分和聚集。结果GC-MS共鉴定出260种挥发性物质,对260个物质进行主成分分析可简化为5个主成分,累积方差贡献率达86.65%,可反映样品的大部分信息;通过主成分分析和聚类分析将6个不同生长时期金银花样品分为4类:其中RB和TG归为1个集群;TW单独归为1个集群,BW和HS归为1个集群;GF单独归为1个集群。结论聚集在一起的金银花样品挥发性物质相似,将RB与TG,TW,BW与HS,GF分为4个不同的商品等级,该研究结果可为金银花混合采摘以及后期的生产加工提供理论依据。

Objective To investigate the differences of volatile substances in different developmental stages of Jinyinhua(Lonicerae Japonicae Flos).Methods Headspace Solid-Phase Microextraction Coupled with Gas Chromatography(HS-SPME-GC-MS) and retention index method were used to analyze the volatile components of Lonicera Japonica in six different stages,including RB,TG,TW,BW,HS and GF,Principal component analysis and cluster analysis were used to determine,distinguish,and aggregate volatile substances of Lonicera Japonica in six different growth stages.Results A total of 260 volatile substances were identified by GC-MS,which could be simplified into five principal components by principal component analysis.The cumulative variance contribution rate of the 260 volatile substances reached 86.65%,which could reflect most of the information of the samples.By principal component analysis and cluster analysis,six samples of Lonicera Japonica at different growth stages were divided into four categories:RB and TG were grouped into a cluster;TW was divided into one cluster,while BW and HS were divided into one cluster.GF was classified as a single cluster.Conclusion The volatile substances of Lonicera Japonica gathered together were similar,RB and TG,TW,BW and HS,and GF were divided into four different commodity grades.The research results can provide a theoretical basis for honeysuckle mixed picking and later production and processing.