补嘘养勒糖浆对产后缺乳模型大鼠治疗作用的研究

Study on therapeutic effect of Buxu Yangle Syrup on bromocriptine mesylate induced hypogalactia in rats

目的探讨补嘘养勒糖浆(BXYL)对甲磺酸溴隐亭诱发模型大鼠缺乳的治疗作用。方法采用甲磺酸溴隐亭复制大鼠缺乳模型,记录缺乳及给药母鼠所喂养仔鼠体重,记录缺乳及给药母鼠泌乳量,酶联免疫吸附测定(ELISA)法检测缺乳及给药母鼠血清泌乳素,病理切片观察缺乳及给药母鼠垂体和乳腺组织形态学变化。结果BXYL高、中剂量组体重及增长率高于模型组,差异有统计学意义(P<0.05)。BXYL高、中、低剂量组分娩后3~10 d的每日8 h泌乳量高于模型组,差异有统计学意义(P<0.01)。BXYL高、中剂量组分娩后第3、7、11天的血清沁乳素水平高于模型组,差异有统计学意义(P<0.05)。与模型组比较,BXYL各剂量组母鼠乳腺腺泡及导管数量明显增多,并扩张增生,乳腺小叶间脂肪结缔组织减少;与模型组比较,BXYL各剂量组母鼠垂体细胞形态正常,嗜酸性细胞增多。结论BXYL对缺乳母鼠有明显的催乳作用。

Objective To investigate the therapeutic effect of Buxu Yangle Syrup(BXYL)on Bromocriptine mesylate induced lactation deficiency in rats.Methods Bromocriptine mesylate was used to establish a rat model of lactation deficiency.The body weight and lactation of the offspring were recorded.The serum prolactin was detected by enzyme linked immunosorbent assay(ELISA).The histopathological changes of hypophysis and mammary gland were observed.Results The body weight and growth rate of the BXYL high and medium dose groups were higher than those of the model group,and the differences were statistically significant(P<0.05).The 8 h lactation per day of the BXYL high,medium and low dose groups on the 3-10 d after delivery were higher than those of the model group,and the differences were statistically significant(P<0.01).The serum quercetin levels in the BXYL high and medium dose groups on the 3rd,7th,and 11th days after delivery were higher than those in the model group,and the differences were statistically significant(P<0.05).Compared with the model group,the number of mammary acini and ducts in each dose group of BXYL increased significantly,expanded and proliferated,and the adipose connective tissue between mammary lobules decreased.Compared with the model group,the morphology of pituitary cells in each dose group of BXYL was normal,and eosinophils increased.Conclusion Buxu Yangle Syrup has obvious effect on promoting lactation in rats with lactation deficiency.