基于宏条形码技术的南海亮眶灯鱼食性初步分析

Preliminary metabacording dietary analysis of Diaphus splendidus in South China Sea

食性研究是鱼类生物学和生态学研究的重要内容,是进行渔业资源开发和管理的基础。以南海亮眶灯鱼(Diaphus splendidus)为研究对象,提取其胃含物DNA,选用线粒体基因细胞色素C氧化酶亚基I(Mitochondrial cytochrome oxidase subunit I,COI)作为分子标记进行扩增,通过高通量测序鉴定其食物组成。结果显示,亮眶灯鱼胃含物中共鉴定出饵料生物34种,隶属于5门7纲11目18科29属。亮眶灯鱼食物组成包括介形类、桡足类、端足类、鱼类、水母类、磷虾类、翼足类和多毛类。其中介形类、桡足类和端足类是其食物组成中的优势类群,这些优势类群与形态学鉴定方法的结果基本一致,此外还检测出形态学方法未鉴定到的水母这一食物类群。研究表明宏条形码技术适用于南海亮眶灯鱼的食性分析,且比基于形态学的食性分析具有更高的鉴别潜力。

Dietary analysis of fish is an important part of fish biology and ecology as well as the basis of fishery resources deve-lopment.In this study,the gastric DNA of Diaphus splendidus was extracted,and the mitochondrial cytochrome c oxidase sub-unit I(COI)was amplified as a molecular marker.The food composition was identified by high-throughput sequencing.The results show that a total of 34 species belonged to 5 phyla,7 classes,11 orders,18 families and 29 genera.The diet of D.splen-didus mainly included Ostracoda,Copepoda,Amphipoda,Fish,Jellyfish,Euphausiacea,Gastropod and Polychaeta.Ostracoda,Copepoda and Amphipoda were the dominant groups in its food composition,which is basically consistent with the previous re-search based on traditional morphological identification,except for jellyfish that was identified only by this study.In conclusion,DNA metabarcoding method is suitable for the food analysis of D.splendidus in the South China Sea,and has higher identifica-tion potential than the food analysis based on morphology.