63份柚类资源S基因型鉴定

Identification of S-genotypes of 63 Pummelo Germplasm Resources

以‘晚白柚’等63份柚类资源为材料基础,通过RNA-seq和序列特征分析发掘新的S-RNase,并结合前期已有的9个S-RNase基因信息,设计21对特异性引物,扩增鉴定63份柚类资源的S基因型。结果发掘到12个新的S-RNase,按顺序分别命名为S_(10)-RNase~S_(21)-RNase。特异扩增结果显示:鉴定的63份材料中,有59份材料得到完整的S基因型,4份仅鉴定到1个S-RNase基因,其中S_(2)-RNase扩增频率最高,达34.92%(22/63),S_(4)-RNase和S_(6)-RNase在63份材料中均未扩增出。结果还显示许多品种具有相同的S基因型。

Based on 63 pummelo germplasm resources,new S-RNases were identified by RNA-seq and sequence analysis.Twenty-one pairs of specific primers were designed to amplify and identify the S-genotypes of 63 pummelo germplasm resources.Results showed twelve new S-RNases were found,named S_(10)-RNase–S_(21)-RNase in sequence.Specific amplification showed that 59 out of 63 materials were identified with complete S-genotype,only one S-RNase gene was identified in four materials.Among them,S_(2)-RNase was amplified in 22 materials with the highest frequency of 34.92%(22/63),while S_(4)-RNase and S_(6)-RNase were not amplified in the 63 materials.The results also showed that many varieties had the same S-genotype.