口腔护理凝胶中人参皂苷Rg1含量的检测方法研究

Study on the Determination Method of Ginsenoside Rg1 Content in Oral Care Gel

建立用高效液相色谱法测定口腔护理凝胶中人参皂苷Rg1含量的方法。采用HPLC法,配置DAD检测器,PG-C18柱(4.6 mm×250 mm,5μm),柱温30℃,以乙腈和水为流动相,经梯度洗脱分离,流速为1.0 mL/min,检测波长203nm,进样量10μL。将口腔凝胶样品用无水乙醇涡旋萃取,再用超声提取30 min,将滤液倒入蒸发皿中,用无水乙醇重复提取一次,合并滤液于蒸发皿中,水浴挥干溶剂后用甲醇定容,注入色谱仪,采用外标一点法定量。平均加样回收率为96.23%(RSD=1.83%)。本方法简便可行,准确可靠,灵敏度高,重现性好,可用于口腔护理凝胶的质量控制。

An analytical method for the determination of ginsenoside Rg1 content in oral care gel has been developed.The determination was performed by high performance liquid chromatography(HPLC)with ultraviolet(DAD)detector.The best chromatographic separation was obtained under the following conditions:PG-C18 column(4.6 mm×250 mm,5μm)set at 30℃and gradient elution of acetonitrile with water as mobile phase pumped at 1.0 mL/min.The detection wavelength was set at 203 nm,and sample volume was 10μL.Oral care gel samples were vortex-extracted by anhydrous ethanol,and then extracted by ultrasonic for 30min.The filtrate was poured into an evaporation dish.Repeat the above extraction once,the filtrate was combined twice and evaporated by water bath.Quantification was carried out by using external standard one point method.The method was satisfactorily applied to 17 samples from different manufactures and batches containing saponins.The spiked recoveries of the ginsenoside Rg1 was 96.23%(RSD=1.83%).Good repeatability and accuracy as well as its handleability make the presented method suitable for the quality control of oral care gel.