树突状细胞与嗜酸性粒细胞在难治性鼻窦炎中的相关性研究

Correlation study between dendritic cell and eosinophil in refractory rhinosinusitis

目的:通过检测难治性鼻窦炎鼻黏膜中树突状细胞的表达和嗜酸性粒细胞浸润情况,探究其与难治性鼻窦炎发病的相关性。方法:对30例正常鼻黏膜、30例慢性鼻窦炎组织和30例难治性鼻窦炎组织分别进行组织病理学染色,检测各组嗜酸性粒细胞浸润情况,免疫组化和免疫荧光双标记检测各组CD83和CD11c表达情况,以单因素方差分析进行多组间差异统计,采用Pearson相关分析测各组CD83和CD11c共表达与嗜酸性细胞浸润的相关性。结果:难治性鼻窦炎组织中嗜酸性粒细胞浸润和树突状细胞的免疫标志物CD83和CD11c的阳性表达与另外两组比较均显著增加(P<0.05),通过Pearson相关性分析,各组CD83和CD11c共表达与嗜酸性粒细胞浸润两者呈正相关关系(P<0.05)。结论:难治性鼻窦炎组织中树突状细胞数目明显增多,且与嗜酸性粒细胞的浸润呈正相关关系,表明树突状细胞和嗜酸性粒细胞在难治性鼻窦炎发生及发展中起着相互促进作用,进一步得出树突状细胞参与了难治性鼻窦炎发生、发展过程。

Objective:To investigate the correlation of refractory rhinosinusitis by detecting the expression of dendritic cells and eosinophils infiltration in nasal mucosa.Methods:Histological staining was performed to detect eosinophils infiltration respectively in 30 normal nasal mucosa,30 chronic rhinosinusitis tissues and 30 refractory rhinosinusitis tissues,Immunohistochemistry and immunofluorescence double labeling were performed to detect the expression of CD83 and CD11c in each group,and univariate anova was used to analyze the differences among groups.The correlation between the co-expression of CD83 and CD11c and the infiltration of eosinophilic cells was measured by Pearson correlation analysis.Results:The positive expression of dendritic cells immune markers CD83 and CD11c and eosinophils infiltration of refractory rhinosinusitis was significantly increased com-pared with the other two groups(P<0.05).Through Pearson correlation analysis,the correlation coefficient r between CD83 and CD11c co-expression and eosinophils infiltration in each group was positive and P<0.05,both were positively correlated.Conclu⁃sion:The number of dendritic cells in refractory rhinosinusitis increased significantly and was positively correlated with the infiltration of eosinophils,which was involved in the development of refractory rhinosinusitis.