摘要
以大肠杆菌(E.coli)作为研究对象,通过标准平板计数法、活死菌染色法以及扫描电子显微镜镜下观察细菌表面结构来研究Ti_(3)C_(2)/Co纳米材料在近红外光照射下的抗菌性能,同时进一步通过溶血实验、脐静脉内皮细胞的cck8细胞毒性试验及迁移试验来验证其生物安全性。结果表明:Ti_(3)C_(2)/Co纳米材料在扫描电子显微镜下呈明显的片层状结构;钴元素的加入使材料表现出优异的磁性性能;Ti_(3)C_(2)/Co复合纳米材料具有良好的抗菌性能,在250μg/mL浓度结合近红外光照射时其杀菌率可达到100%;而且Ti_(3)C_(2)/Co复合纳米材料在一定浓度范围内表现出优异的细胞相容性。材料具有潜在的广阔应用前景,可以制成抗菌涂层应用于医疗导管、手术器械及防菌纱布中。
This experiment took E.coli as the research target flora.The antibacterial properties of Ti_(3)C_(2)/Co nanomaterials under near-infrared light(NIR)were studied by standard plate counting,live-dead bacteria staining,and observation of the bacterial surface structure under a scanning electron microscope.Hemolysis test,cck8 cytotoxicity test and migration test of umbilical vein endothelial cells were further verified its biological safety.The results showed that Ti_(3)C_(2)/Co nanomaterials were clear lamellar structures.The magnetic cobalt nanowires endowed Ti_(3)C_(2)/Co nanomaterials with controllable magnetic ability.Ti_(3)C_(2)/Co composite nanomaterials had good antibacterial properties,and its sterilization rate could reach 100%at a concentration of 250μg/mL when combined with near-infrared light.At the same time,Ti_(3)C_(2)/Co composite nanomaterials showed excellent cell compatibility within a certain concentration range.This material had potential application prospects and could be used as an antibacterial agent in many fields such asmedical catheters,surgical instruments and antibacterial gauze.
作者
周璇
郑云飞
贾绮林
张斐然
ZHOU Xuan;ZHENG Yunfei;JIA Qilin;ZHANG Feiran(School of Stomatology,Peking University,Beijing 100081,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2022年第3期30-35,共6页
Journal of Biology
基金
国家自然科学基金青年项目(81700938)。