肠杆灵散及球虫血痢散对鸡肠道微生物菌群结构多样性影响比较试验研究

The effect of Changganling powder and coccidia dysentery powder on chicken intestinal microflora comparative experimental study on the influence of structural diversity

本试验通过Pacbio高通量宏基因组测序分析,研究“球虫血痢散”和“肠杆灵散”兽用中草药组方药物应用对鸡肠道菌群结构的影响,并与禽肠道常用抗生素药物硫酸安普霉素进行比较。通过设置3个药物试验组和1个空白对照,连续7 d用药,收集4个试验组鸡大肠段内容物样品,经总DNA提取、PCR扩增及高通量宏基因组序列测定分析。结果显示:“球虫血痢散”和“肠杆灵散”组方药物试验组Shannon指数显著高于硫酸安普霉素组和空白对照组,且组间差异显著(P﹤0.05),其中“球虫血痢散”组方药物试验组ACE值为60.09,表明肠道菌群多样性优势突出;基于Weighted Unifrac距离的PCoA分析结果显示,各药物试验组间菌落构成差异较小,但与空白对照组菌落构成差异较大。在门和种水平上,3个药物试验组中变形杆菌门(Proteobacteria)均呈显著下降趋势,厚壁菌门(Firmicutes)占比上升趋势明显,同时各试验药物对降低鸡埃希氏-大肠杆菌(Escherichia-coil)的比例均优于空白对照组且差异显著(P﹤0.05),表明各试验药物均有利于鸡肠菌群平衡紊乱的改善。其中“肠杆灵散”药物组在提高Streptococcus(非解乳糖链球菌FGM)的丰度占比上要优于“球虫血痢散”组,但硫酸安普霉素组的微生物种类含量比例显著低于2个兽用中草药药物试验组,且均匀性低。因此,2个兽用中草药组方药物在抑制鸡埃希氏-大肠杆菌增殖的同时,更有利于鸡肠道菌群平衡紊乱的改善和维持菌群结构的多样性。

In this study,Pacbio high-throughput metagenomic sequencing analysis was used to study the effects of Chinese herbal medicine prescriptions of“coccidiosis and dysentery powder”and“Changganling powder”on the intestinal flora structure of chickens.Three drug experimental groups and one blank control group were set up,continuous medication for 7 days,and collected the contents of chicken large intestine in four experimental groups.Through the total DNA extraction,PCR amplification and high-throughput macrogenomic sequencing.the results showed that the Shannon index of“Changganling powder”drug group and“coccidia dysentery powder”group was significantly higher than that of the ampramycin sulfate group and the blank control group(P<0.05).The ACE value of the drug test group of“coccidia dysentery powder”was 60.09,indicating that the diversity of intestinal flora was prominent.The results of PCoA analysis based on weighted Unifrac distance showed that there was little difference in the colony composition among the experimental groups,but compared with the control group,the colony composition was significantly different,At the phylum and species levels,Proteobacteria in the three drug test groups showed a significant downward trend,and Firmicutes showed a significant upward trend.At the same time,the test drugs on reducing the proportion of chicken Escherichia-coil were better than the blank control group and the difference was significant(P<0.05),indicating that the test drugs were beneficial to improve the imbalance of chicken intestinal flora.Among them,the“Changganling powder”drug group was better than the“coccidia dysentery powder”group in increasing the abundance of Streptococcus(non-lactolytic streptococcus FGM),but the proportion of microbial species in ampramycin sulfate group was significantly lower than that in two veterinary Chinese herbal medicine test groups,and the uniformity was low.Therefore,the two the proliferation of chicken E.coli,the two veterinary herbal prescriptions were more conducive to the improvement of intestinal flora balance disorder and the maintenance of diversity of flora structure.